Process for producing a culture of antrodia camphorata and product obtained thereby
A kind of technology of culture, culture medium, be applied in in order to manufacture Antrodia camphorata culture and the product field obtained by described, can solve problems such as Antrodia camphorata expensive
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Embodiment 1
[0063] Embodiment 1: the preparation of Antrodia camphorata liquid culture
[0064] The original culture of Antrodia camphorata isolate CCRC 930032 was maintained at -80°C, a small amount of fungi were removed from the original culture, and placed on a potato dextrose agar plate medium (PDA, purchased from Difco). After the fungi were rejuvenated, the cultures were transferred to potato dextrose agar slants. The slant medium was grown at 25°C and subcultured every two months. The slants are intended for use as working cultures. To prepare mycelial inoculum, cultures derived from PDA slants were inoculated on PDA plates and incubated at 28°C for 15 to 20 days.
[0065] Preparation of mycelium inoculum
[0066] The fungi were cultured until a mycelial colony with a diameter of 15 to 30 mm was observed. The mycelial features of Antrodia camphorata were examined under a light microscope to ensure that they were not contaminated. Cut the whole mycelium into small pieces, and t...
Embodiment 2
[0067] Example 2: Effect of Shaking Rate on the Pharmacological Activity of Fungal Filtrates
[0068] 2.7 liters of synthetic medium (2% fructose, 0.5% (w / v) yeast extract (DIFCO), 0.1% (w / v) KH 2 PO 4 (Merck) and 0.05% (w / v) of MgSO 4 ·7H 2 O (Merck)), and then the Antrodia camphorata CCRC 930032 inoculum source obtained in Example 1 was added to the synthetic medium at a volume ratio of 1:9. The culture was grown at 30°C with an aeration rate of 0.6 liters / minute. The culture shaking rate was initially set at about 200 rpm and increased to about 500 rpm after 74 hours of incubation. Several samples were taken from the culture at 48, 113, 170, 217 and 259 hours after inoculation of the mycelium, and then passed through a simple filter consisting of a filter funnel, conical flask and aspirator device to remove most of the insoluble matter. Ammonia (NH 4 OH) Adjust the pH value of the obtained filtrate to 7, and perform moist heat sterilization. The obtained samples wer...
Embodiment 3
[0079] Example 3: Effect of pH on the Pharmacological Activity of Fungal Filtrates
[0080] Three synthetic medium batches (1.5% fructose, 0.5% (w / v) yeast extract ( DIFCO), 0.1% (w / v) of KH 2 PO 4 (Merck) and 0.05% (w / v) of MgSO 4 ·7H 2 O (Merck)), and the inoculum source prepared in Example 1 was added to these synthetic media at a volume ratio of 1:9. The resulting cultures were grown as described in Example 2, but the pH of each culture was monitored at predetermined time points and carefully adjusted to around the initial pH by addition of NaOH solution (Table 2). The incubation procedure lasted 336 hours. The timing of adding NaOH solution depends on the selected pH value. For example, the NaOH solution was added from the 192nd and 168th hour to maintain the pH at about 4.9 to 5.1 and about 5.4 to 5.6 in Test B and Test C, respectively, while the NaOH solution was not added until the 240th hour in Test A.
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