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Method for changing IL-2 receptor distribution in T cell film micro area and its use

A cell membrane and micro-region technology, applied to cells modified by introducing foreign genetic material, active ingredients of anhydride/acid/halide, drug combination, etc.

Inactive Publication Date: 2005-10-19
NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the role of PUFAs on IL-2R signaling in membrane fat microdomains

Method used

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  • Method for changing IL-2 receptor distribution in T cell film micro area and its use
  • Method for changing IL-2 receptor distribution in T cell film micro area and its use
  • Method for changing IL-2 receptor distribution in T cell film micro area and its use

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Experimental program
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Embodiment 1

[0025] 1. Experimental materials

[0026] cis-4,7,10,13,16,19-Docosahexaenoic acid (22:6 n-3) and stearic acid (18:0) were purchased from Sigma-Aldrich (St, Louis, MO). Human Jurkat E6-1 T cells (American Type Culture Collection, Bethesda, MD). RPMI 1640 medium, newborn bovine serum, serum-free IMDM medium (Iscove's Modified Dulbecco's Medium) were obtained from Invitrogen (Grand Island, NY). Human interleukin-2 (hIL-2), bovine serum albumin (component V), protease inhibitor cocktail and BM chemiluminescence Western blotting kit were purchased from Roche (Indianapolis, IN). Goat anti-human IL-2Rα, IL-2Rβ and IL-2Rγ for westem-bloting c Antibodies were products of R&D Company (Minneapolis, MN). Rabbit anti-goat IgG (H&L) horseradish peroxidase conjugated antibody was obtained from Chemicon International (Temecula, CA).

[0027] 2. Cell culture and fatty acid treatment:

[0028] Human Jurkat E6-1 T cells (American Type Culture Collection, Bethesda, MD) were added to RPMI 1...

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Abstract

The present invention discloses the method of changing IL-2 receptor distribution in T cell membrane micro areas and its application. The method processes IL-2 stimulated T cell with fatty acid. The method is simple, and can change the IL-2 receptor distribution in T cell membrane micro areas effectively to make the IL-2 receptor shift from the T cell membrane fat micro areas to soluble membrane component. The method may be used in preparing immunity inhibitor.

Description

technical field [0001] The invention relates to a method for changing the distribution of receptors in T cell membrane micro-area, in particular to a method for changing the distribution of IL-2 receptors in T-cell membrane micro-area, and the application of the method in the preparation of immunosuppressants . Background technique [0002] Lipid rafts are functional micro-regions within the liquid lipid bilayer of the cell membrane, rich in glycolipids, sphingomyelin and cholesterol, and are cell-membrane micro-regions that are insoluble in nonionic active agents. Simons et al. (2000) identified the role of membrane fat microdomains in cell signaling, arguing that many important receptors and cell signaling proteins are located as their constituents or inducibles in the fat microdomains where signaling processes are accomplished , the compartmentalization of this receptor signaling molecule is functionally important. Recent findings suggest that important molecules of man...

Claims

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Application Information

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IPC IPC(8): A61K31/185A61P37/06C12N5/10
Inventor 李秋荣黎介寿
Owner NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A