Process for preparing chondroitin sulfate

A technology of chondroitin sulfate and cartilage, which is applied in the field of preparation of chondroitin sulfate, can solve the problems of limited product production capacity and scale, chondroitin sulfate cannot be effectively intercepted, and the recovery rate of products is not ideal, so as to reduce the safety Hidden danger, low cost, white color effect

Inactive Publication Date: 2005-11-23
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new technology doesn't involve any steps like removing unwanted substances from sugar beets before they are being processed for extraction purposes. It produces products that look whiter without losing their original taste due to its lack of chemical reactions involved when processing them. Additionally, it uses less expensive materials than traditional methods while still producing high-quality sugars at an affordable level. Overall, this innovative technique simplifies the manufacturing processes by eliminating unnecessary waste material and increasing yields compared to existing techniques.

Problems solved by technology

This patents describes different ways to produce chondaranic acid (chondroitan) from bacteria called Actinosynnema sanguinea or Micrococcus luteus. These processes involve various steps such as salting out calcium-containing substances like sodium carbonates by adding water followed by extraction into organics solvents containing acids at specific temperatures. After this step, it can then react further under certain conditions resulting in desired compounds.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0018] 1. Put 500Kg of clean cartilage into the reaction tank, add an appropriate amount of deionized water until the cartilage is completely submerged, and cook at 100°C for 6 hours;

[0019] 2. Add 5Kg of sodium sulfite as a protective agent to the above-mentioned cooking treatment solution, and prepare a solution with a concentration of 1-2% of 5Kg of sodium hydroxide, add it to the above-mentioned cooking treatment solution, and protect and leaching at 50°C for 8 hours;

[0020] 3. Take the leachate, adjust the pH to 8.5, add 5Kg trypsin, and enzymolyze at 50°C for 8 hours;

[0021] 4. Heat the above materials to 85°C for 40 minutes, filter after heat treatment, and filter out protein precipitation;

[0022] 5. Use a hollow fiber ultrafilter and an ultrafiltration membrane with a cut molecular weight of 1000-3000 to perform ultrafiltration purification on the filtrate obtained in process 4. Use the concentration mode first, and then use the concentration-dialysis mode to c...

Embodiment 2

[0026] Embodiment 2: This embodiment is basically the same as the foregoing embodiment, except that the protective agent used is potassium nitrite, and the strong base is potassium hydroxide.

Embodiment 3

[0027] Embodiment 3: This embodiment is basically the same as the foregoing embodiment, except that the protective agent used is sodium borohydride, and the strong base is potassium hydroxide.

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Abstract

The invention relates to a process for preparing chondroitin sulfate which comprises boiling treatment, protecting or mild leaching, enzymolysis, filtering and removing protein, ultrafiltration purifying, vacuum concentrating, spraying and seasoning, the yield is between 5-35%.

Description

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Claims

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Application Information

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Owner SHANGHAI UNIV
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