Detection of transcription factor protein by double chain RNA molecule fixed to coated micro-plate
A technology of transcription factor and double-stranded nucleic acid, which is applied in the field of double-stranded nucleic acid molecule-coated microwell plate to detect transcription factor protein, can solve the problems of limiting the commercialization of double-stranded DNA microarray chip technology, high cost of chip preparation and use, etc.
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[0047] Here, only the experiments of preparing double-stranded nucleic acid molecules to coat microwell plates and detecting the transcription factor protein NF-κB are used to illustrate the specific implementation of the patent of the present invention.
[0048] 1. Design and chemical synthesis of double-stranded nucleic acid molecules
[0049] NF-κB (Nuclear Factor kappaB) is a sequence-specific transcription factor. When cells are stimulated by various substances such as inflammatory mediators, virus infection, and oxidative stress, NF-κB is activated in the cytoplasm, enters the nucleus and interacts with viruses. , cytokines, growth factors, cell adhesion molecules, acute phase response proteins, enzymes and other gene enhancer sequences combined to enhance gene transcription; thus play a role in the pathogenesis of a series of diseases involving cytokines, inflammatory mediators and proteases important role. A large number of studies have shown that NF-κB hyperactivatio...
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