Detection of transcription factor protein by double chain RNA molecule fixed to coated micro-plate

A technology of transcription factor and double-stranded nucleic acid, which is applied in the field of double-stranded nucleic acid molecule-coated microwell plate to detect transcription factor protein, can solve the problems of limiting the commercialization of double-stranded DNA microarray chip technology, high cost of chip preparation and use, etc.

Inactive Publication Date: 2006-01-18
王进科 +1
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Problems solved by technology

Although our double-stranded DNA microarray chip technology has reached the practical level, we noticed that the popularization and application of this technology still faces great difficulties, that is, the cost of chip preparation and use is relatively high
In particular, the actual use of the chip must rely on the current very expensive gene chip scanning device, which greatly limits the commercialization of double-stranded DNA microarray chip technology

Method used

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  • Detection of transcription factor protein by double chain RNA molecule fixed to coated micro-plate
  • Detection of transcription factor protein by double chain RNA molecule fixed to coated micro-plate

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Embodiment Construction

[0047] Here, only the experiments of preparing double-stranded nucleic acid molecules to coat microwell plates and detecting the transcription factor protein NF-κB are used to illustrate the specific implementation of the patent of the present invention.

[0048] 1. Design and chemical synthesis of double-stranded nucleic acid molecules

[0049] NF-κB (Nuclear Factor kappaB) is a sequence-specific transcription factor. When cells are stimulated by various substances such as inflammatory mediators, virus infection, and oxidative stress, NF-κB is activated in the cytoplasm, enters the nucleus and interacts with viruses. , cytokines, growth factors, cell adhesion molecules, acute phase response proteins, enzymes and other gene enhancer sequences combined to enhance gene transcription; thus play a role in the pathogenesis of a series of diseases involving cytokines, inflammatory mediators and proteases important role. A large number of studies have shown that NF-κB hyperactivatio...

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Abstract

Transcription factor is one important protein for gene regulation, is center of gene expression regulating passage and network, is important target of functional genome and protein block research and important target site of transcription treatment and medicine research owing to that many diseases are closely related to the abnormal expression and activation of transcription factor. The present invention proposes double stranded nucleic acid molecule coated in microporous plate to detect transcription factor protein. The process of analyzing transcription factor expression and activation level includes the following steps: preparing nucleic acid molecule; fixing the nucleic acid molecules to the pores of the microporous plate; incubation of cell or tissue extract containing transcription factor in the plate; incubation of transcription factor antibody in the plate; incubation of chemically marked transcription factor diantibody on the plate; and detecting and analyzing the chemical marker.

Description

1. Technical field: [0001] Transcription factor (transcription factor) is a kind of important protein responsible for gene expression regulation in proteomics. ) and proteome research; many diseases are closely related to the abnormal expression and activation of transcription factors, and have become important targets for transcriptional therapy and drug research (drug target). The detection and analysis of transcription factor expression and activation degree is the main means to study its function. This patent proposes a new method for detecting the expression and activation level of transcription factors, which will be used in the research of transcription factors in the fields of molecular biology, functional genomics, proteomics and biomedicine Provide a new detection and analysis technology that can promote scientific research on transcription factors in these fields, and provide a diagnostic technology for disease-related transcription factors in the biomedical field,...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 王进科
Owner 王进科
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