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Methods of tangential flow filtration and an apparatus therefor

A filter membrane and flow rate technology, applied in the field of tangential flow filtration and its devices, can solve the problems of difficulty in precise particle size control and success

Inactive Publication Date: 2006-04-12
GTC BIOTHERAPEUTICS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0016] But precise control of the particle size required for the technology to be used commercially has been difficult and not generally successful

Method used

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  • Methods of tangential flow filtration and an apparatus therefor
  • Methods of tangential flow filtration and an apparatus therefor
  • Methods of tangential flow filtration and an apparatus therefor

Examples

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Embodiment 1

[0095] Milk is used as a feed solution for the production of target molecules

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Abstract

Processes and apparati are provided for separating molecules of interest from a mixture containing them which comprises subjecting the mixture to an improved method of tangential flow filtration (TFF). The improved TFF was used to clarify, and process various feedstreams for the removal of a molecule of interest. According to a preferred embodiment, a transgenic milk feedstream is stabilized and particulate matter such as fat, casein miscelles and bacteria are removed. The method of TFF used in the current invention utilizes optimized process parameters that include temperature, transmembrane pressure, cross-flow velocity, and milk concentration. Cleaning and storage procedures were also developed to ensure long membrane life. An aseptic filtration step was also developed to remove any bacteria remaining in a clarified transgenic milk feedstream.

Description

field of invention [0001] The present invention provides improved methods and systems for isolating specific target molecules from contaminants. More particularly, the present invention relates to the treatment of sample solutions by an improved tangential flow filtration process which improves the clarification, concentration and fractionation of desired molecules from a given feed solution separate. Background of the invention [0002] The present invention relates to an improved method of filtering target molecules from a given feedstream. It should be noted that the production of large quantities of relatively pure, biologically active molecules is very important for the economical and efficient production of human and animal pharmaceutical formulations, proteins, enzymes, antibodies, and other specialty chemicals. Recombinant DNA technology has become the method of choice for the production of many kinds of polypeptides, antibodies and proteins, because a large number...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C07K1/34
CPCC07K1/34
Inventor D·E·克托A·拉沃蒂尔
Owner GTC BIOTHERAPEUTICS INC
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