Nolvac viral coat protein with recombinant rod virus expression and its preparation

Abstract

NLVs rBJV with recombinant rod virus expression and its production are disclosed. One is NLVs rBJV, another is open reading gene recombinant rod virus expression protein with 1623 bases and 540 coded amino acid residue. The process is carried out by designing cloning primer, extracting virus RNA, reverse transcripting, enlarging, cloning, sequence determining, oriented sub-cloning, purifying plasmid, co-transfecting, screening, purifying recombinant virus, expressing rBJV, and purifying recombinant virus particle. It can be used to build up enzyme-linked immunosorbent assay and serum investigation and research on gene engineering oral vaccine with NLVs virus infection prevention.

Description

technical field [0001] The invention relates to Norwalk-like virus (Norwalk-likeviruses, NLVs) capsid protein (recombinant Beijing virus, rBJV) expressed by recombinant baculovirus and a preparation method thereof, in particular to the second open reading of Norwalk-like virus Capsid protein expressed by frame gene recombinant baculovirus and preparation method thereof. Background technique [0002] Norwalk-like virus is one of the main pathogens causing acute gastroenteritis. It causes the outbreak and distribution of acute gastroenteritis in children and adults through water, food, etc., among which raw shellfish food is the cause of acute gastroenteritis. The most common cause of viral outbreaks. From 1987 to 1992, among the outbreaks of acute gastroenteritis in the Kyushu area of ​​Japan, 4 times were confirmed to be related to eating raw oysters. Schwab et al. used reverse transcription polymerase chain reaction to confirm that it can accumulate in shellfish organisms...

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Problems solved by technology

[0005] Since Norwalk-like virus cannot be cultured in cells and there is no suitable animal model, only a small amount of viral antigen can be o

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