Duck herpesvirus type 1 ul7 truncated recombinant protein and its preparation method and application

A technology of recombinant protein and duck herpes, which is applied in the field of bioengineering, can solve the problems of difficult purification of virus antigens, time-consuming and labor-intensive operations, and difficulty in standardization, etc., and achieves the effect of being suitable for large-scale industrial production, facilitating the comparison of results, and simple preparation methods
CN104072591BActive Publication Date: 2016-06-08SICHUAN AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SICHUAN AGRI UNIV
Publication Date
2016-06-08

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Abstract

The invention discloses an anatid herpesvirus 1 (AHV-1) UL7 truncated recombinant protein shown in SEQ ID No.2, as well as a code gene, a recombinant expression vector and engineering bacteria of the AHV-1 UL7 truncated recombinant protein, and a method for preparing the truncated recombinant protein and a polyclonal antibody of the truncated recombinant protein from the engineering bacteria. The truncated recombinant protein is similar to a natural AHV-1 UL7 protein in immunoreactivity, can be specifically bound with an AHV-1 antibody to be served as a reagent for detecting the AHV-1 antibody. As the truncated recombinant protein is not a complete bacteria, the risk of poison diffusion can be avoided during detection; the preparation method is simple, and suitable for industrialized mass production; product standardization can be realized; comparison among results of different laboratories can be facilitated; the polyclonal antibody of the truncated recombinant protein can serve as a reagent for detecting an AHV-1 antigen, is high in specificity, cannot react with duck hepatitis virus and new type gosling enteritis virus in a crossed manner, and can serve as reagent application for positioning and distributing the UL7 protein in an AHV-1 infected host cell.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering, and relates to a virus truncated recombinant protein and its preparation method and application. Background technique

[0002] Anatid Herpesvirus 1 (AHV-1) belongs to Herpesviridae, α-Herpesvirinae, and Marekvirus genus, which can cause an acute, febrile, contact-infected infection in waterfowl such as ducks, geese, and swans. Bloody infectious disease, the clinical manifestations of sick ducks are depression, high fever, ataxia, diarrhea, tearing and swelling of the head and neck of some sick ducks. The main clinical feature is the process of acute sepsis. The main habitat of wild waterfowl spreads rapidly, and the morbidity and mortality can be as high as 90%. It often causes serious economic losses and seriously threatens the survival and development of my country's waterfowl breeding industry. AHV-1 antigen or antibody detection is critical to its prevention and treatment. The main met...

Claims

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