Duck plague virus ul15 gene exoni recombinant protein and its preparation method and application

A duck plague virus and recombinant protein technology, which is applied in the field of biochemistry, can solve the problems of easy to disperse, lack of generalization, and unsatisfactory purity, and achieves the effect of strong specificity
CN104292310BActive Publication Date: 2017-10-31SICHUAN AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SICHUAN AGRI UNIV
Publication Date
2017-10-31

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Abstract

The invention discloses a duck plague virus UL15 gene exonI recombinant protein as well as a preparation method and application thereof. The amino acid sequence of the duck plague virus UL15 gene exonI recombinant protein is as shown in SEQ ID NO.4. The preparation method comprises the following steps: overnight culturing escherichia coli Rossetta containing a recombinant expression vector in an Amp-containing LB liquid culture medium; and transferring a bacterium solution into a fresh Amp-containing LB liquid culture medium, culturing until OD600 is 0.4, and adding IPTG for induced culture to obtain a culture solution of the duck plague virus UL15 gene exonI recombinant protein. The recombinant protein has immunocompetence, can be specifically combined with the antibody of duck plague virus, can be used as a detection reagent of duck plague virus antibody or used as an antigen for preparing a polyclonal antibody, so that a foundation is laid for detection of duck plague virus.
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Description

technical field

[0001] The invention belongs to the field of biochemistry, and in particular relates to a duck plague virus UL15 gene exonI recombinant protein, and also relates to a preparation method and application of the recombinant protein. Background technique

[0002] Duck plague (Duck Plague, DP) is an acute, febrile, contact-infected septicemia infectious disease of waterfowl such as ducks, geese and swans caused by duck plague virus (DPV) in the Herpesviridae family. . The disease can lead to a decrease in egg production and death of commercial waterfowl, and it also has a different lethality rate to wild waterfowl. In recent years, with the intensive and large-scale development of the duck industry, duck plague, as one of the most serious contagious diseases threatening the duck industry, has caused huge economic losses.

[0003] Clinical and laboratory tests have confirmed that attenuated duck plague virus vaccine is an effective biological agent for the preven...

Claims

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