Chinese crab in-vitro hatching technology

A Chinese mitten crab technology, which is applied in the field of Chinese mitten crab nursery, can solve the problems of high cost, high environmental pressure, and low survival rate of crab seedlings, and achieve the advantages of less water consumption, high seedling production rate, and labor saving. Effect

Inactive Publication Date: 2006-08-16
王吉桥 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the technical problems of high cost, high environmental pressure and low survival rate of crab seedlings in the technology, and provides a Chinese mitten crab in vitro hatching and seedling technology that can save seedling raising costs and improve the quality of edible crabs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] a. Take the live oviposit crabs after the blastula stage, such as the survival oviposit crabs in the blastula stage, gastrula stage, eye point stage and heartbeat stage;

[0019] b. Turn the ventral surface of the oviposit crab upwards, gently lift the abdomen with hands, and cut off the abdominal limb attached to the egg from the bottom segment with surgical scissors. The abdomen runs through the digestive tract. When cutting the embryos, the opening of the abdomen should be small so that it can be cut; if the opening is too large, cutting too deep will damage the digestive tract and cause parent crabs to die;

[0020] c. Hang it on the metal support (egg hanging device) after being sterilized by permanganate A;

[0021] d. Inflate the embryos for hatching and larvae cultivation, the water temperature is 16.0°C to 25.0°C, the salinity is 30.0 to 34.0, the water is continuously inflated, and the dissolved oxygen is at least 4.5mg / l. When the stage I daphnia (Z 1 ), fee...

Embodiment 2

[0023] Others are the same as in Example 1, the difference is that the cut abdominal limbs are placed in water with a water temperature of 6-8°C and a salinity of 30.0-34.0 for immersion, and the isolated embryos are preserved. The time required by the larvae determines the hatching and cultivation time of the embryo. The effective accumulated temperature of river crabs from fertilization to hatching of larvae is 220°C.d.

Embodiment 3

[0025] Others are the same as in Example 1 or Example 2, except that the cut abdominal limbs are cut into small sections again, and the size of the small sections should be fixed on the support for convenience, so as to increase the contact area between the embryo and the fresh water body. During the hatching of isolated embryos, the water source must be strictly filtered to ensure clean water quality, sufficient dissolved oxygen, and moderate aeration; the temperature difference should not exceed 5°C; Soak the cut embryos with Chinese herbal medicine.

[0026] time

[0027] When the oviposit crabs with the embryos cut off were reared in the cage for 12-15 days, it was found that there were black marks on the cut off abdomen and no inflammatory reaction. Both parent crabs with embryos cut and control crabs with uncut embryos gained 3-2 g in weight. Anatomical observation found that the ovaries of most parent crabs whose eggs were cut off were blue-purple and darker...

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PUM

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Abstract

An in vitro incubation and culture technique for Eriocheir japonica sinensis includes such steps as providing the living costegite crab, shearing out costegopod, hanging it from supporting frame, aerating while incubating in the water at 16-25 deg.C with salinity of 30-34, and culturing the larvae while controlling water quality.

Description

Technical field: [0001] The invention relates to a seedling raising technology of Chinese mitten crab, in particular to an in vitro hatching seedling raising technology of Chinese mitten crab which can save seedling raising costs and improve the quality of edible crabs. Background technique: [0002] Chinese mitten crab (Eriocheir sinensis, commonly known as river crab), in the 1990s, successfully cultivated seedlings with natural seawater and artificial seawater, making the river crab from wild to fully artificial breeding. Mature female river crabs have a wide hind abdomen, and the 2nd to 5th pairs of abdominal limbs are double-branched, with many and long bristles, which are used to attach and hold egg groups. The setae are born in the setae fossa (joint cavity) formed by the triangular membrane, and are connected externally between the outer egg membrane and the middle layer. In the genus of river crabs, the middle yellow eggs, the fertilized egg and the parent body are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A61D19/00
CPCY02A40/81
Inventor 王吉桥张涛张剑诚王文辉
Owner 王吉桥
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