A probiotic composition comprising at least two lactic acid bacterial strains which are able to colonise the gastrointestinal tracts in combination with having intestinal survival property, intestinal
A technology of protection characteristics and probiotics, applied in the direction of bacteria used in food preparation, medical preparations containing active ingredients, Lactobacillus, etc., can solve the problems of inability to survive, temporarily settle, and not adhere to the colon mucosa.
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[0022] All LAB-strains of different species were grown aerobically on MRS agar at 37°C for 24 hours. In further experiments, growth at different temperatures was determined. All strains can reproduce at temperatures from +4°C to 40°C or 45°C. All strains produced protease as determined by skim milk agar assay (24 hours, aerobic, 37°C). None of the strains produced nitrite or nitrate.
[0023] All LAB-strains of different species could utilize prebiotics, such as inulin and pullulan, but not β-glucose, as determined by growth on YNB medium utilizing fibers such as prebiotics as the sole carbon source. glycans. Since the strains are able to ferment these fibers, they will play a beneficial role in the colonic flora. Enhanced fiber degradation also increases crude fiber digestibility.
[0024] In the composition of the invention, a mixture of cocci and bacilli is optimal because cocci and bacilli have different generation times and bacilli proliferate much faster than cocci....
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