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Technological process of extracting lactoferritin Lf from human foremilk and its product

A lactoferrin and extraction process technology, applied in the field of separation and extraction of lactoferrin Lf, can solve problems such as no reports, and achieve the effects of overcoming cancer pain, formulating scientifically and rationally, and improving activity

Inactive Publication Date: 2006-10-18
蒋来高
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] At present, the technology of extracting and isolating homologous and high-purity lactoferrin from human colostrum has not been reported

Method used

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Embodiment Construction

[0040]The present invention will be described below in conjunction with three examples of the extraction process of human colostrum lactoferrin Lf. In the three extraction process embodiments, except for the parameters listed in Table 1, all the others are the same as the following process steps.

[0041] The human colostrum lactoferrin Lf extraction process comprises the following steps:

[0042] 1. Take excess colostrum from healthy puerperas, and use PH value, specific gravity, sensory and other inspection methods to control the quality of raw materials;

[0043] 2. Centrifuge to remove cells and fat;

[0044] 3. Centrifuge to remove fat, and refrigerate below -25°C;

[0045] 4. Pasteurization, at 63.5°C, after 30 minutes, kills microorganisms and viruses;

[0046] 5. After thawing the frozen skim milk at room temperature, curd the milk to remove the casein;

[0047] 6. Crush the clot at 4°C, centrifuge at 7000r / min for 20 minutes to remove the precipitate, and collect ...

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PUM

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Abstract

The present invention provides extracting process and product of high purity lactoferritin (Lf) from human foremilk. The extracting process includes the following steps: collecting human foremilk of health parturient, centrifuging to eliminate impurity and fatty, eliminating casein from curd, collecting the supernatant as the lactalbumin, processing with 300PS membrane, vacuum concentrating, and freeze drying to obtain extracted Lf with purity of 98 %. The high purity Lf product includes Lf and additive, which may be immunoglobulin, amino acid, Chinese herbal medicine, biological preparation, milk product or their composition, and may be in tablet, capsule or oral liquid.

Description

technical field [0001] The invention belongs to the technical field of separating and extracting lactoferrin Lf from human breast milk colostrum by using bioengineering technology. In particular, it relates to an extraction process of human colostrum extract lactoferrin Lf and a product thereof. Background technique [0002] 1. Basic structure of lactoferrin [0003] Lactoferrin is one of the main glycoproteins in the mammary gland secretions of mammals (human, cow, horse, sheep) in the stages of colostrum formation, lactation, and lactation. It is a protein with multiple biological functions. It not only participates in the transport of iron, but also has the functions of antimicrobial, anti-oxidation, anti-tumor, powerful pain relief and regulating the immune system. It is considered to be a new type of antibacterial and antiviral , anticancer pain relief drugs and food and feed additives with great development potential. [0004] Lactoferrin, also known as "globin" bec...

Claims

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Application Information

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IPC IPC(8): C12P21/00
Inventor 蒋来高
Owner 蒋来高
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