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High-efficiency mycoplasma quantitative culture medium and preparation process thereof

A liquid culture medium and culture medium technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as clinical diagnosis errors, inability to quantitatively detect mycoplasma, and abuse of antibiotics, and achieve quantitative and accurate results.

Inactive Publication Date: 2006-12-13
SHANGHAI UPPER BIO TECH PHARMA
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  • Summary
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  • Description
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AI Technical Summary

Problems solved by technology

[0041] In addition, there are many different formulas such as basal medium, liquid medium, and solid medium, which are all applied to the isolation and cultivation of mycoplasma in clinical and scientific research, but the common disadvantage of the existing technology is that it can only be used for qualitative detection and cannot Quantitative detection of mycoplasma in clinical significance
Because the mycoplasma test of normal people also shows positive results, it directly leads to clinical diagnosis errors and the abuse of antibacterial drugs in clinical treatment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Weigh 20g of PPLO culture medium, add 980mL of distilled water, then add about 8.0ml of 1N HCL, adjust the pH to 6.2-6.3, heat to dissolve, divide into Erlenmeyer flasks, and sterilize under high pressure at 121°C for 20min;

[0066]Weigh 0.02 g of ampicillin, add distilled water to dissolve it, add it to the above-mentioned culture medium, add to the above-mentioned culture medium, dry it, and sterilize it under high pressure at 121° C. for 20 minutes.

[0067] Weigh 25g of L-arginine and 10.9g of urea, add 100ml of distilled water and 20ml of 2N HCL to dissolve, and sterile filter with a 0.22μm filter membrane.

[0068] Weigh 16g of yeast extract, add 130ml of distilled water, sterilize at 121°C for 20min, and mix with the above solution after autoclaving. Add 350ml of horse serum and 12ml of 1% phenol red, shake well. Weigh 2.52g of ampicillin and 10ml of sterile water, dissolve it and add it to the medium above. Add 3.7ml each of SMZ (3400mg / ml) solution. Add 2N ...

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Abstract

The invention relates to efficient mycoplasmal medium, especially to a urea agent and human mycoplasmal efficient quantitative medium and its process for preparing, which includes (1) basal culture medium (2) fluid medium and mainly consists of mycoplasmal PPLO, arginine, aquacare, acillin and SMZ. The said medium can perform quantitative determination for genital tract mycoplasmal positive result, can guide the clinical diagnosis and treatment, and also have important significance in preventing abuse of antibacterial drugs.

Description

technical field [0001] The invention relates to a mycoplasma culture medium, in particular to a high-efficiency mycoplasma high-efficiency quantitative culture medium and a preparation method thereof. Background technique [0002] Mycoplasma is a common pathogen of reproductive tract, respiratory tract and cell strain infection, accounting for 30%-50% of the entire infectious disease. Nongonococcal urethritis is the most common STD in the world, second only to gonorrhea. There are as many as seven kinds of mycoplasma detected from the human genitourinary tract, including mycoplasma hominis, ureaplasma urealyticum, and mycoplasma genitalium. In sexually transmitted and genitourinary tract diseases, the detection rate of mycoplasma as the pathogen accounts for 40%-70% of the STD population. There is also a small amount of mycoplasma in the urogenital tract of normal human body, but it does not cause disease. After a lot of research, it is currently internationally recognized...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
Inventor 徐建新
Owner SHANGHAI UPPER BIO TECH PHARMA
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