Turtle germplasm identification method based on mtDNA
An identification method, the technology of Chinese soft-shelled turtle, applied in the field of germplasm identification of Chinese soft-shelled turtle based on mtDNA, can solve the problems of reduced morphological differences between strains, resource degradation, germplasm pollution, etc., and achieve accurate germplasm identification without unproblematic evidence Effect
Inactive Publication Date: 2006-12-20
吴惠仙 +1
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Problems solved by technology
In recent years, with the expansion of market demand and the rapid development of the soft-shelled turtle industry, along with the introduction of foreign species and the non-origin breeding of domestic strains, the phenomenon of hybridization between strains, germplasm pollution and resource degradation has occurred. The morphological differences between the latter lines are reduced, making it difficult to identify germplasm based on morphological characteristics
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[0028] Such as figure 1 As shown, a kind of mtDNA-based soft-shelled turtle germplasm identification method of the present invention comprises following two big steps:
[0029] 1. To establish the mtDNA-based germplasm identification standard of Chinese soft-shelled turtle, which specifically includes the following ten sub-steps:
[0030] 1.1. Sample collection and identification: Take the original species with significant germplasm characteristics of each strain of soft-shelled turtle, and collect fresh blood, crony, and muscle samples. The amount of each sample is 0.5-1.0 grams. Use directly or store in a -20°C refrigerator stand-by;
[0031] 1.2. Total DNA extraction: use SDS and proteinase K digestion, and phenol / chloroform method to extract whole genome DNA (this is the most basic and least expensive extraction method, and other extraction methods can also be used);
[0032] 1.3. DNA quality detection: measure the concentration and purity of DNA with an ultraviolet spec...
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The invention discloses a Chinese soft-shelled turtle quality authenticating method based on mtDNA, which comprises the following steps: collecting and authenticating sample; extracting total DNA, detecting DNA quality; augmenting object gene segment; detecting augmented product; testing sequence of object gene; correcting sequence; building standard molecular system tree for Chinese soft-shelled turtle quality; collecting mtDNA sequence of detected Chinese soft-shelled turtle; comparing each kind system corresponding sequence of detecting sequence with the built Chinese soft-shelled turtle quality; establishing new system tree; analyzing akin relationship; judging strain of detected Chinese soft-shelled turtle. The invention provides more reasonable and precise strain detecting method without non-specific evidence problem due to morphology elementary.
Description
technical field [0001] The invention relates to an animal germplasm identification method, in particular to an mtDNA-based soft-shelled turtle germplasm identification method. Background technique [0002] The traditional germplasm identification of soft-shelled soft-shelled turtle is based on the morphological characteristics of soft-shelled turtle. In recent years, with the expansion of market demand and the rapid development of the soft-shelled turtle industry, along with the introduction of foreign species and the non-origin breeding of domestic strains, the phenomenon of hybridization between strains, germplasm pollution and resource degradation has occurred. The morphological differences between the latter lines were reduced, making it difficult to identify germplasm based on morphological characteristics. Contents of the invention [0003] The technical problem to be solved by the present invention is to propose an mtDNA-based identification method for Chinese soft...
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Inventor 吴惠仙薛俊增徐雪娜罗天生
Owner 吴惠仙