Extracting method and use of ainsliaea fragrans champ-extract

A kind of rabbit ear wind and extract technology, which is applied in the field of extraction of rabbit ear wind extract, and can solve the problems of outdated preparation method and low content of active ingredients, etc.

Inactive Publication Date: 2007-01-24
陈正道
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The preparation process of the current commercially available rabbit ear wind preparation is outdated, and the active ingredient content is low (the total organic acid content is about 10%)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0013] Experimental Example 1: Trial of three extraction methods

[0014] Alcohol extraction method: take 1Kg of rabbit ear wind, extract twice with 95% ethanol, combine the extracts, recover ethanol, dry to obtain ethanol paste, and weigh. The yield is about 5%. 1g of extract is equivalent to 20g of crude drug; it is F1.

[0015] Water extraction and alcohol precipitation method: Take 1Kg of rabbit ears, cut into sections or crush, boil and extract 3 times with 10 times the amount of heated water, 2 hours each time, filter, combine the filtrates for 3 times, add ethanol to make the alcohol content reach 70%, static Set aside for 12 hours, filter, recover ethanol, and dry to obtain 263 g of ethanol paste. 1g of extract is equivalent to 3.8g of crude drug; it is F2.

[0016] The macroporous adsorption resin method of the present invention: the purification of the organic acid components of the rabbit ear wind is carried out by using the macroporous adsorption resin. Take 1K...

experiment example 2

[0017] Experimental example 2: Identification of the chemical structure of the extract of Xingxiang rabbit ear wind

[0018] Take 1kg of the whole herb of Xingxiang rabbit's ear wind, chop it up, extract it with water three times, each time for 1 hour, concentrate it under reduced pressure to 6 liters, centrifuge the supernatant and pass it through 1kg of macroporous resin. 95% ethanol elution. After concentration and drying, 30 g of rabbit ear wind extract (that is, F3 in the above experimental example) was obtained. 1 g of the extract powder was separated by high-speed countercurrent chromatography and collected according to the chromatographic peaks to obtain compounds A1 (21 mg) and A2 (26 mg).

[0019] High-speed countercurrent chromatography separation conditions: separation with solvent system chloroform:methanol:water=2:2:1, detection wavelength: 254nm, flow rate: 3ml / min, pressure: 8 kgf.

[0020] Compound A 1 : Light yellow amorphous powder (methanol), the chemica...

experiment example 3

[0022] Experimental example 3: Determination of the chemical composition and properties of the extract of apricot rabbit ear wind

[0023] Take an appropriate amount of the extract, configure it into a solution with a certain concentration with methanol, and detect it with a high performance liquid phase with a diode array detector. The two highest compounds A1 and A2 were identified as organic acids, so the main components of the rabbit ear wind extract were organic acids.

[0024] Chromatographic conditions: Waters high-performance liquid chromatography (autosampler, 600 pump, 2996 ultraviolet diode array detector); octadecyl silica gel bonded phase packed column; mobile phase: methanol-water (42:58). Preparation of reference substance solution: Accurately weigh 2.04mg of A2 reference substance, dilute it in a 2mL volumetric flask with methanol, and shake well to obtain a reference substance stock solution with a concentration of 1.02mg / mL.

[0025] Preparation of the test ...

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PUM

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Abstract

The present invention discloses a kind of glabrous ainsliaea herb extract, which is extracted with glabrous ainsliaea herb, and through crushing, decocting in distilled water, filtering, concentrating filtrate, regulating pH with hydrochloric acid, centrifuging, adsorbing the supernatant in macroporous resin, eluting with ethanol, merging with acid precipitate and drying. The present invention also discloses the application of the glabrous ainsliaea herb extract in preparing medicine for treating acute and chronic cervicitis.

Description

technical field [0001] The invention relates to an extraction method and a new application of a medicinal extract, in particular to an extraction method and a new application of a rabbit ear wind extract. Background technique [0002] Apricot-fragrant rabbit ear wind (Ainsliaea fragrans Champ.) is a plant of the genus Ainsliaea fragrans Champ. Apricot Fragrant Rabbit Ear Wind is sweet in nature and flavor, slightly bitter, cool in nature, and returns to the lung and liver channels. Have heat-clearing and invigorating deficiency, cooling blood for hemostasis, promoting diuresis and detoxification. Indications for consumptive bone steaming, tuberculosis and hemoptysis, metrorrhagia in women, damp-heat jaundice, edema, carbuncle and swelling, scrofula and tuberculosis, bruises, snake bites. Cervical Erosion Treated Clinically with Xingxiang Rabbit Ear Wind Preparation and Folk Proof [3，4] , endometritis [5] , heat furuncle [6] ,thrush [7] . As a seldom-used traditional C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61K9/48A61K9/20A61K9/16A61K9/08A61K9/10A61K9/02A61P15/00
CPCY02A50/30
Inventor 陈正道
Owner 陈正道
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