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Peptide stabilization

A stable, stable quantity technology, applied in the field of proteins and peptides, can solve problems such as unsatisfactory stability

Inactive Publication Date: 2007-04-18
BIOHIT OYJ
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Due to the problems posed by the unsatisfactory stability of not only gastrin-type peptides, but also many other proteins and peptides, including hormones and enzymes, efforts have been made to find means of stabilizing these sensitive analytes

Method used

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Examples

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Embodiment Construction

[0012] The stabilizer used in accordance with the present invention is the organic solvent dimethylsulfoxide, DMSO; which is known for various industrial, medical and laboratory uses. The molecular weight of DMSO is 78.1, it is miscible with water, its melting point is 18.5°C, and its boiling point is 189°C. It is a by-product of the paper industry, where it is derived from lignin, the binding substance in trees.

[0013] In cell culture, a 10% solution in DMSO is routinely used when freezing cells. In this application, DMSO acts as a protective agent to reduce osmotic damage, since cells are very sensitive to intracellular ice formation. DMSO quickly penetrates cell membranes and displaces water within them. Therefore, DMSO also penetrates the skin rapidly and can easily transport components dissolved therein, such as low molecular components, across the skin. To this end, DMSO is used as an adjuvant to facilitate the absorption and transport of drug molecules. It has als...

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PUM

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Abstract

The present invention is directed to the use of DMSO for the stabilization of temperature sensitive peptides, namely gastrin and gastrin related peptides, against degradation in a mammalian biological sample, such as a serum or plasma sample.

Description

field of invention [0001] The present invention generally relates to the field of stabilizing temperature-sensitive analytes, especially proteins and peptides, including hormones and enzymes, from degradation in biological samples, such as plasma or serum samples from mammals. Background of the invention [0002] One problem encountered in the analysis of analytes in biological samples, especially serum and plasma samples, involves the poor stability of some proteins and peptides. Small peptides are especially susceptible to enzymatic degradation. Poor stability requires that samples be tested immediately, or stored in a low temperature environment, such as a refrigerator. However, even under low temperature storage, the stability is often insufficient. If such samples are to be stored for many days, they must be packed in dry ice. This is of course quite inconvenient. [0003] Gastrin-17 is a peptide hormone secreted by gastric antrum G cells u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/595G01N33/68C07K
CPCG01N2333/595C07K14/595
Inventor 奥斯摩·索瓦尼米奥利·林纳拉
Owner BIOHIT OYJ