K-ras oligonucleotide microarray and method for detecting K-ras mutations employing the same

Abstract

Since the K-ras oligonucleotide microarray of the present invention can detect K-ras mutations by applying a competitive DNA hybridization method to the oligonucleotides spotted on a solid matrix different from the previously reported method for detecting a mutation, it makes the more precise analysis and can reduce experimental cost and time. Accordingly, the K-ras oligonucleotide microarray of the present invention can be used in studies to detect K-ras mutations and unravel the signal transduction mechanism and tumorigenesis related to K-ras gene. Further, since the microarray of the present invention can be applied to other genes having mutational hot spot regions such as K-ras, it has wide applicable range.

Description

technical field

[0001] The invention relates to a K-ras oligonucleotide microarray for detecting mutations in K-ras gene mutation hot spots, a manufacturing method thereof and a method for detecting K-ras mutations. Background technique

[0002] K-ras is one type of ras gene that is mutated in various cancers. Mutations in the K-ras gene at codons 12 and 13 are involved in tumorigenesis by causing a functional modification of the p21-ras protein, a K-ras gene product, that transmits excessive growth signals to the nucleus, stimulating cell growth and division. The incidence of K-ras mutation is about 90% in pancreatic cancer, about 50% in colorectal cancer, and about 30% in non-small cell lung cancer. The mutation profile shows that about 85% of mutations Occurs at codons 12 and 13 (Samowitz WS et al., Cancer Epidemiol. Biomarkers Prev. 9: 1193-1197, 2000). Therefore, the identification of K-ras gene mutations has been widely used as a useful tool i...

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