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Process for extracting beta-mannanase utilizing Erwinia

A technology of mannanase and Erwinia, applied in the direction of hydrolytic enzymes, etc., can solve the problems of slow industrial development, high production costs, and late start of research, and achieve the effect of enhancing competitiveness

Inactive Publication Date: 2011-09-21
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0029] The research on the breeding of mannanase high-yielding strains at home and abroad started relatively late and achieved relatively little results. Therefore, although the market prospect of mannanase is very good, the industrial development is relatively slow due to the relatively high processing costs.
At the same time, compared with textile and papermaking industries, hemp degumming and grass pulping are only a preliminary process of extracting fibers from herbal fiber raw materials. If the production cost of high-efficiency bacterial agents is too high, it will inevitably lead to a lack of market price for the final product. Competitiveness in terms of

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  • Process for extracting beta-mannanase utilizing Erwinia
  • Process for extracting beta-mannanase utilizing Erwinia

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Embodiment Construction

[0086] (1) Technical solution

[0087] The technical solution involved in the present invention includes technological steps (processes) such as original seed activation, seed cultivation, fermentation, protease inhibition, rough separation, microfiltration, ultrafiltration, salting out, dissolution, column chromatography, formulation, and packaging.

[0088] 1. Activation of original species

[0089] The original seed is activated, and the typical colony of CXJZ95-198 is inoculated into the ordinary glucose nutrient broth medium, shaken at 120r / min-180r / min, 33°C-35°C for 5.0h-5.5h to obtain the seed bacterial liquid.

[0090] 2. Primary seed cultivation

[0091] Inoculate the activated CXJZ95-198 seed bacterium liquid into a laboratory aeration stirring fermenter, the medium is configured with tap water, and the formula is: the concentrations of glucose, konjac powder, peptone, beef extract, and NaCl are 0.5%, 0.2%, 0.5%, 0.5%, 0.5%, natural pH value, inoculum size 2%, tem...

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Abstract

This invention involves the technology of extraction of beta-mannan enzyme from Erwinia bacilli in the industry of biological agents. The technical program includes original species activation, primary seed cultivation, secondary seed cultivation, fermentation, proteinase inhibitor, crude separation, refining of concentrated efficient bacilli and mannan-liquid, formulas, vacuum packaging technology and condensed efficient liquid agent and mannan enzyme products. This invention uses centralized preparation agent program to reduce technical difficulty of bio-degumming and biological pulping process, and can reduce the production cost by extracting cell pellets and secretions from the same broth. The efficient concentrated liquid agent, produced by this invention technical programs, is suitable for bio-degumming of crudefiber plants, biological pulping of grasses and biological glycosylated processing of herbaceous fiber raw materials biopulping alcohol; mannan enzymes are widely used in food, medicine, fodder, paper, textiles, petroleum mining industry.

Description

1. Technical field [0001] The invention relates to a process for simultaneously preparing bacterial agents and enzyme preparations from the same fermented liquid through diversion. Bacteria agents are mainly used for the extraction and refining of herbal fibers, including hemp biodegumming in the textile industry, biopulping in the paper industry, and biosaccharification of herbal fiber raw materials in the alcohol production industry; β-mannanase is used in food, medicine, and feed , papermaking, textile printing and dyeing, petroleum exploration and biotechnology research have been widely used. 2. Background technology [0002] (1) Development status and trends at home and abroad [0003] 1. The development of herbal fiber resources is a global development trend [0004] With the shortage of oil and forest resources and the improvement of the quality of human life, the domestic and foreign textile and paper industries are developing an upsurge in the development and util...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/24
Inventor 刘正初胡镇修冯湘沅彭源德张运雄段盛文郑科
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI