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Instrument for detecting bacterium, method of detecting bacterium and kit for detecting bacterium

A technology for bacterial detection and kits, applied in the fields of botanical equipment and methods, biochemical equipment and methods, biochemical instruments, etc., can solve the problem of requiring multiple days for identification

Inactive Publication Date: 2007-07-04
SUNTORY HLDG LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The most important thing in the management of food microbes is to provide accurate test results for the food manufacturing site in a timely manner. However, with the commonly used detection methods and classification identification methods, the identification of microorganisms takes many days. For example, the identification of microorganisms almost takes about several days~ 1 week

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0215] [Synthesis of oligonucleotide]

[0216] According to a prescribed method, oligonucleotides were synthesized using an oligonucleotide synthesizer (manufactured by Perkin-Elmer Applied Biosystems), deprotected, and then dried. The dried oligonucleotide was dissolved in 10 mM Tris-HCl (pH 7.5) and 1 mM EDTA buffer to prepare a 100 pmol / µL oligonucleotide solution. All oligonucleotides used in this example were synthesized by this method.

[0217] The base sequences of the synthesized oligonucleotides are represented by SEQ ID NOs: 1 to 57, wherein SEQ ID NOs: 1 to 40 and 43 to 57 represent capture oligonucleotides, and SEQ ID NOs: 41 and 42 represent primers. For the capture oligonucleotide, the amino group was bound to the 5' end using the synthesizer described above, whereas for the primer, biotin was bound to the 5' end.

[0218] [Spotting capture oligonucleotides on the substrate]

[0219] 10 µL of the microspotting solution (manufactured by TeleChem International I...

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PUM

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Abstract

The present invention provides a bacteria detection device, bacteria detection method and bacteria detection kit enabling rapid and accurate determination of bacteria present in a test sample, and particularly spore-forming, aerobic bacteria, with a simple procedure. The bacteria detection device according to the present invention is a microarray type of device in which an oligonucleotide, which is based on a nucleotide sequence specific to a genus or species to which a target spore-forming, aerobic bacteria belongs, is immobilized on a substrate. Spore-forming, aerobic bacteria present in a test sample can be easily, rapidly and accurately detected and identified based on the presence or absence of successful hybridization between a probe prepared from the test sample and the oligonucleotide immobilized on the substrate.

Description

technical field [0001] The present invention relates to a bacterium detection device, a bacterium detection method and a bacterium detection kit, more specifically, to a bacterium detection device in which specific oligonucleotides of aerobic spore-forming bacteria are immobilized on a substrate, and an extremely Accurate and operable bacteria detection method and bacteria detection kit. Background technique [0002] Conventionally, in the food industry, various hygiene management techniques have been researched and developed in order to improve the safety of manufactured food. Especially in recent years, the safety of food has been strictly regulated, and interest in improving technology is also increasing. [0003] When sanitation management is carried out at the food manufacturing site, microbiological management is necessary, so it is necessary to detect and identify microbes. The detection and identification of microorganisms requires professional knowledge and techno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12M1/34C12N15/00C12Q1/04
CPCC12Q1/689C12Q1/6834
Inventor 山中实喜子守屋彰悟小佐野薰桶川高史
Owner SUNTORY HLDG LTD