Universal stem cells

a stem cell and universal technology, applied in the field of universal stem cells, can solve the problems of further complexity, cell effect, and the possibility of haplotype- and isotype-mismatched dimers, and achieve the effect of cells
US20060037092A1Inactive Publication Date: 2006-02-16LAWMAN MICHAEL J P +1
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
LAWMAN MICHAEL J P
Publication Date
2006-02-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The subject invention pertains to materials and methods for preparing multi-potential stem cells having a pre-selected expression of MHC antigens. Stem cells of the subject invention can be used to generate histocompatible tissues / organs for transplantation. The process of the subject invention comprises the use of targeting vectors capable of gene knockout, insertion of site-specific recombination cassettes, and the replacement of histocompatibility alleles in the stem cell. Novel knockout vectors are used to delete designated regions of one chromosome. Recombination cassette vectors are then used to delete the same region on the second chromosome and deposit a site-specific recombination cassette which can be utilized by replacement vectors for inserting the new MHC genes on the chromosome of the engineered cell. The subject invention also pertains to cells, tissues, and transgenic mammal prepared using the methods and materials of the invention.
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Description

CROSS-REFERENCE TO RELATED APPLICATION

[0001] This application is a divisional application of U.S. patent application Ser. No. 10 / 186,231 filed Jun. 28, 2002 which is a continuation of U.S. patent application Ser. No. 09 / 047,769 filed Mar. 25, 1998, now abandoned, which claims the benefit of U.S. Provisional Application No. 60 / 042,358, filed Mar. 25, 1997.BACKGROUND OF THE INVENTION

[0002] The immune systems of mammals have evolved to protect the organism from infectious diseases. Included in the mammalian immune system are many cells that carry out this function by a complex combination of soluble messengers, receptors, adhesion molecules, recognition molecules and signals. Interactions between these components are quite elaborate and take place at specific sites within the organism as well as the primary site of infection. In order to protect the organism from the invasion of viruses, bacteria and other microorganisms, the immune system must be able to distinguish what is self (ho...

Claims

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