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Compounds and methods for treatment and diagnosis of chlamydial infection

a technology of chlamydia and compound, which is applied in the field of detection and treatment of chlamydia, can solve the problems of tubal obstruction, infertility, and chlamydia thus constituting a significant health problem

Inactive Publication Date: 2008-07-31
CORIXA CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chlamydia trachomatis is one of the most common causes of sexually transmitted diseases and can lead to pelvic inflammatory disease (PID), resulting in tubal obstruction and infertility.
Chlamydial infections thus constitute a significant health problem both in the US and worldwide.
For example, by the time a woman seeks medical attention for PID, irreversible damage may have already occurred resulting in infertility.

Method used

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  • Compounds and methods for treatment and diagnosis of chlamydial infection
  • Compounds and methods for treatment and diagnosis of chlamydial infection
  • Compounds and methods for treatment and diagnosis of chlamydial infection

Examples

Experimental program
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example 1

Isolation of DNA Sequences Encoding Chlamydia Antigens

[0754]Chlamydia antigens of the present invention were isolated by expression cloning of a genomic DNA library of Chlamydia trachomatis LGV II essentially as described by Sanderson et al. (J. Exp. Med., 1995, 182:1751-1757) and were shown to induce PBMC proliferation and IFN-γ in an immunoreactive T cell line.

[0755]A Chlamydia-specific T cell line was generated by stimulating PBMCs from a normal donor with no history of chlamydial genital tract infection with elementary bodies of Chlamydia trachomatis LGV II. This T cell line, referred to as TCL-8, was found to recognize both Chlamydia trachomatis and Chlamydia pneumonia infected monocyte-derived dendritic cells.

[0756]A randomly sheared genomic library of Chlamydia trachomatis LGV II was constructed in Lambda ZAP (Stratagene, La Jolla, Calif.) and the amplified library plated out in 96 well microtiter plates at a density of 30 clones / well. Bacteria were induced to express recombi...

example 2

Induction of T Cell Proliferation and Interferon-γ Production by Chlamydia Trachomatis Antigens

[0770]The ability of recombinant Chlamydia trachomatis antigens to induce T cell proliferation and interferon-γ production is determined as follows.

[0771]Proteins are induced by IPTG and purified by Ni-NTA agarose affinity chromatograph (Webb et al., J. Immunology 157:5034-5041, 1996). The purified polypeptides are then screened for the ability to induce T-cell proliferation in PBMC preparations. PBMCs from C. trachomatis patients as well as from normal donors whose T-cells are known to proliferate in response to Chlamydia antigens, are cultured in medium comprising RPMI 1640 supplemented with 10% pooled human serum and 50 μg / ml gentamicin. Purified polypeptides are added in duplicate at concentrations of 0.5 to 10 μg / mL. After six days of culture in 96-well round-bottom plates in a volume of 200 μl, 50 μl of medium is removed from each well for determination of IFN-γ levels, as described ...

example 3

Preparation of Synthetic Polypeptides

[0778]Polypeptides may be synthesized on a Millipore 9050 peptide synthesizer using FMOC chemistry with HPTU (O-Benzotriazole-N,N,N′,N′-tetramethyluronium hexafluorophosphate) activation. A Gly-Cys-Gly sequence may be attached to the amino terminus of the peptide to provide a method of conjugating or labeling of the peptide. Cleavage of the peptides from the solid support may be carried out using the following cleavage mixture: trifluoroacetic acid:ethanedithiol:thioanisole:water:phenol (40:1:2:2:3). After cleaving for 2 hours, the peptides may be precipitated in cold methyl-t-butyl-ether. The peptide pellets may then be dissolved in water containing 0.1% trifluoroacetic acid (TFA) and lyophilized prior to purification by C18 reverse phase HPLC. A gradient of 0-60% acetonitrile (containing 0.1% TFA) in water (containing 0.1% TFA) may be used to elute the peptides. Following lyophilization of the pure fractions, the peptides may be characterized u...

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Abstract

Compounds and methods for the diagnosis and treatment of Chlamydial infection are disclosed. The compounds provided include polypeptides that contain at least one antigenic portion of a Chlamydia antigen and DNA sequences encoding such polypeptides. Pharmaceutical compositions and vaccines comprising such polypeptides or DNA sequences are also provided, together with antibodies directed against such polypeptides. Diagnostic kits containing such polypeptides or DNA sequences and a suitable detection reagent may be used for the detection of Chlamydial infection in patients and in biological samples.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation of U.S. patent application Ser. No. 10 / 872,155 filed Jun. 18, 2004; which is a continuation of U.S. patent application Ser. No. 09 / 841,132 filed Apr. 23, 2001 (now abandoned); which is a continuation-in-part of U.S. patent application Ser. No. 09 / 620,412, filed Jul. 20, 2000 (now U.S. Pat. No. 6,448,234); which is a continuation-in-part of 09 / 598,419, filed Jun. 20, 2000 (now U.S. Pat. No. 6,565,856); which is a continuation-in-part of U.S. patent application Ser. No. 09 / 556,877, filed Apr. 19, 2000 (now U.S. Pat. No. 6,432,916); which is a continuation-in-part of U.S. patent application Ser. No. 09 / 454,684, filed Dec. 3, 1999 (now abandoned); which is a continuation-in-part of U.S. patent application Ser. No. 09 / 426,571, filed Oct. 22, 1999 (now abandoned); which is a continuation-in-part of U.S. patent application Ser. No. 09 / 410,568, filed Oct. 1, 1999 (now U.S. Pat. No. 6,555,115); which is a continu...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/00A61K31/70A61K38/00A61P37/00G01N33/53A61K31/7088A61K39/118A61K48/00A61P31/00A61P37/04A61P43/00C07K14/295C07K16/12C07K19/00C12N1/15C12N1/19C12N1/21C12N5/06C12N5/10C12N15/09C12N15/31C12Q1/06G01N33/566G01N33/569
CPCA61K38/00A61K39/00A61K48/00A61K2039/505C07K2319/00A61K2039/515A61K2039/5154A61K2039/53C07K14/295A61K2039/51A61P31/00A61P31/04A61P37/00A61P37/04A61P43/00A61K39/4622A61K39/4611A61K39/4615A61K39/464835A61K38/16
Inventor BHATIA, AJAYSKEIKY, YASIR A. W.PROBST, PETER
Owner CORIXA CORP
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