36 results about "Ethanedithiol" patented technology

The present invention relates to a method for solid-phase synthesis of atosiban, comprising the following steps: 1) taking Rink Amide resin as a carrier, and obtaining H2N-Rink Amide resin after removing Fmoc-protection; 2) using HOBT and DIPCI as condensation reagents, and The carboxyl group of Fmoc-Gly-OH is connected with the amino group of the resin to obtain Fmoc-Gly (resin); 3) adopt the Fmoc strategy to synthesize the remaining amino acids in the solid phase sequence sequentially; 4) carry out solid phase cyclization with iodine; 5) then use the cleavage reagent ( Trifluoroacetic acid / thioanisole / 1,2-ethanedithiol / water) cleavage and ether precipitation to obtain crude atosiban peptide; 6) the crude product was prepared and separated by HPLC to obtain pure atosiban.

The invention relates to a reagent for cutting peptides, in particular to a reagent for cutting sulfydryl-containing peptides from resins. In addition, the invention also relates to a method for cutting sulfydryl-containing polypeptides from resins, which is suitable for industrial production of sulfydryl-containing polypeptides. The reagent for cutting sulfydryl-containing polypeptides from resins comprises the following components in parts by weight: 85-95 parts of trifluoroacetic acid, 1-5 parts of triisopropylsilane, 1-4 parts of mercaptoundecanoic acid, 1-4 parts of phenol and 1-2 parts of water. The invention has the advantages that because ethanedithiol is a chemical reagent which is not produced at home, the cost of the ethanedithiol is expensive, and the ethanedithiol has effluvial smell in the reaction process; in the invention, mercaptopropionic acid is used for replacing the conventional ethanedithiol, thus the production cost can be lowered, the environment pollution is greatly reduced, and simultaneously, the effect of the peptide cutting reaction can also be improved; and meanwhile, the invention has the characteristics of low procurement cost, reduction of the three wastes, good crude peptide quality and the like, and is very suitable for industrial production of sulfydryl-containing polypeptides.

A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.