Immunotherapy for reversing immune suppression
a technology of immune suppression and immunotherapy, applied in the field of immunotherapy and vaccine immunotherapy, can solve the problems of sporadic and generally minor successful efforts, inability to consistently carry out immunization, and inability to reverse tumor regression, so as to promote differentiation and maturation of immature dendritic cells, and restore t cell immunity.
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[0116]All steps relating to cell culture are performed under sterile conditions. General methods of cellular immunology not described herein are performed as described in general references for cellular immunology techniques such as Mishell and Shiigi (Selected Methods in Cellular Immunology (1981)) and as are known in the art.
[0117]Preparation of Natural Cytokine Mixture (NCM)
[0118]The buffy coat white cells of human blood from multiple HIV-negative hepatitis virus-negative donors is collected. In an alternative embodiment, animals are the cell source for veterinary uses. The cells from the donors are pooled and layered on ficoll hypaque gradients (Pharmacia) to yield lymphocytes free of neutrophils and erythrocytes. Alternative methods could be used that would result in the same starting lymphocyte population as are known in the art.
[0119]The lymphocytes are washed and distributed in X vivo-10 media (Whittaker Bioproducts) to surface activated cell culture flasks for selection of ...
example 2
[0164]There is shown that local perilymphatic injections in the neck having NCM plus low dose cyclophosphamide, indomethacin, and zinc induced clinical regressions in a high percentage of patients with squamous cell head and neck cancer (H&NSCC) (Hadden J W, et al., Arch Otolaryngol Head Neck Surg 120:395-403 (1994); Meneses A, et al., Arch Pathol Lab Med 122:447-454 (1998); Barrera J, et al., Arch Otolaryngol Head Neck Surg 126:345-351 (2000)) with evidence of improved, recurrence-free survival. Overall, including minor response (25%-50%) tumor shrinkage and reduction of tumor in pathological specimens, over 90% responded and the majority had greater than 50% tumor reduction.
[0165]These responses were speculated to be mediated by immune regression since both B and T lymphocytes were observed infiltrating the tumors. The therapy was not associated with significant toxicity.
[0166]Several unpublished observations serve to document this speculation and lead to the present invention.
[01...
example 3
[0176]Two patients were treated with lymphoma of the head and neck. The patients included were those with head and neck cancer who agreed to participate in the protocol. The following scheme was followed:
[0177]Before treatment, the patients were skin-tested with NCM 0.1 ml subcutaneously in the forearm, the region was marked, and 24 hours later, the test was read. The test was considered positive if the induction and erythema was equal or larger than 3 mm.
[0178]Each cycle of NCM was for 21 days, as follows:
Day 1Low dose cyclophosphamide (300 mg / m2 i.v.)Days 1-21Indomethacin 25 mg p.o. 3 times dailyZinc sulfate 50 mg p.o. once dailyDays 3-12NCM 200 units 5 as 1 ml subcutaneouslyperilymphatic in the neck
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