Compositions and methods for modulating pten expression

a composition and pten technology, applied in the field of oligonucleotide based compositions, can solve the problems of uncontrolled cell growth, morbidity and mortality, etc., and achieve the effect of reducing or enhancing the expression of pten, relieving or preventing the repression of pten by prc2

Inactive Publication Date: 2015-06-11
RANA THERAPEUTICS INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0005]Aspects of the invention disclosed herein provide methods and compositions that are useful for upregulating PTEN in cells. In some embodiments, single stranded oligonucleotides are provided that target a PRC2-associated region of a PTEN gene (e.g., human PTEN) and thereby cause upregulation of the gene. In some embodiments, single stranded oligonucleotides are provided that target a PRC2-associated region of the gene encoding PTEN. In some embodiments, t

Problems solved by technology

Cancer includes a multitude of diseases characterized by uncontrolled growth of cells in an individual, which can ultimately result in morbidity and mortality.
Mut

Method used

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  • Compositions and methods for modulating pten expression
  • Compositions and methods for modulating pten expression
  • Compositions and methods for modulating pten expression

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[0198]The invention is further described in the following examples, which do not limit the scope of the invention described in the claims.

Materials and Methods:

Real Time PCR

[0199]RNA was harvested from the cells using Promega SV 96 Total RNA Isolation system or Trizol omitting the DNAse step. In separate pilot experiments, 50 ng of RNA was determined to be sufficient template for the reverse transcriptase reaction. RNA harvested from cells was normalized so that 50 ng of RNA was input to each reverse transcription reaction. For the few samples that were too dilute to reach this limit, the maximum input volume was added. Reverse transcriptase reaction was performed using the Superscript II kit and real time PCR performed on cDNA samples using icycler SYBR green chemistry (Biorad). A baseline level of mRNA expression for each target gene was determined through quantitative PCR as outlined above. Baseline levels were also determined for mRNA of various housekeeping genes which are cons...

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Abstract

Aspects of the invention provide single stranded oligonucleotides for activating or enhancing expression of PTEN. Further aspects provide compositions and kits comprising single stranded oligonucleotides for activating or enhancing expression of PTEN. Methods for modulating expression of PTEN using the single stranded oligonucleotides are also provided. Further aspects of the invention provide methods for selecting a candidate oligonucleotide for activating or enhancing expression of PTEN.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims the benefit under 35 U.S.C. §119(e) of U.S. Provisional Application No. 61 / 785,885, entitled “COMPOSITIONS AND METHODS FOR MODULATING PTEN EXPRESSION”, filed Mar. 14, 2013 and U.S. Provisional Application No. 61 / 648,041, entitled “COMPOSITIONS AND METHODS FOR MODULATING PTEN EXPRESSION”, filed May 16, 2012, each of which is incorporated herein by reference in its entirety.FIELD OF THE INVENTION[0002]The invention relates to oligonucleotide based compositions, as well as methods of using oligonucleotide based compositions for treating disease.BACKGROUND OF THE INVENTION[0003]Cancer includes a multitude of diseases characterized by uncontrolled growth of cells in an individual, which can ultimately result in morbidity and mortality. In 2008, over 7.6 million people died from cancer worldwide, accounting for approximately 13% of all deaths in the world. Cancer is one of the highest priority public health concerns, wit...

Claims

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Application Information

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IPC IPC(8): C12N15/113
CPCC12N15/1137C12N2310/31C12N2310/351C12N2310/3231C12N2310/321C07H21/00C07H21/02C07H21/04C12Y301/03016C12Y301/03048C12Y301/03067C12N15/1135C12N2310/11C12N2310/315C12N2310/346A61K31/7115A61K31/712A61K31/7125A61P35/00A61P43/00C12N2310/3521
Inventor KRIEG, ARTHUR M.SUBRAMANIAN, ROMESHMCSWIGGEN, JAMESLEE, JEANNIE T.
Owner RANA THERAPEUTICS INC
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