Process for producing itaconic acid and itaconic acid esters under anaerobic conditions

Inactive Publication Date: 2018-08-30
DSM IP ASSETS BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The invention is about the discovery of cells that can produce itaconic acid and its ester under anaerobic conditions, specifically yeast cells. Using yeast cells is advantageous because they are tolerant to low pH and are not filamentous, which makes it easier to produce the desired chemicals. An anaerobic process also has higher productivity and yield compared to an aerobic process, while requiring less energy for mixing and cooling.

Problems solved by technology

However, Aspergilli are less suitable for industrial production of itaconic acid due to its filamentous morphology, leading to oxygen transfer problems in large scale bioreactors.
This approach is problematic, however, since E. coli, and prokaryotes in general, are not tolerant to low pH.
This in turn leads to increased DSP costs since recovery of the acid from the salt is more complex, as compared with a low pH fermentation process, where the acid can be directly recovered from the fermentation broth by crystallization.
However, the modified Y. lipolytica does not produce significant amounts of itaconic acid on sugar, one of the most commonly available renewable feedstocks.

Method used

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  • Process for producing itaconic acid and itaconic acid esters under anaerobic conditions
  • Process for producing itaconic acid and itaconic acid esters under anaerobic conditions
  • Process for producing itaconic acid and itaconic acid esters under anaerobic conditions

Examples

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Effect test

example 1

ssion of Enzymes for Different Metabolic Pathways for Itaconic Acid and Itaconate Methyl Ester Production in Saccharomyces cerevisiae

[0311]1.1 Expression Constructs

[0312]The nucleotide sequences of SEQ ID NOs 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, and 47 are obtained by the codon-pair optimization method as disclosed in PCT / EP2007 / 05594 for S. cerevisiae were synthesized. The nucleotide sequences of SEQ ID NOs 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63 and 64 were synthesized. From these sequences (promoter, open reading frame and terminators) expression cassettes were built according to the methods described in the co-pending patent application no. U.S. 61 / 616,254 and WO2013 / 144257. The formed expression cassettes (cassette 117-cassette 149) were used as a template to PCR amplify the DNA fragments used in the transformation.

[0313]1.2 Preparation and Purification of PCR Fragments for Transformation

[0314]Assembly and in...

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Abstract

The present invention relates to a method for the production of itaconic acid or an ester of itaconic acid, which method comprises fermenting a recombinant cell capable of producing itaconic acid or an ester of itaconic acid in a suitable fermentation medium under anaerobic conditions, wherein the itaconic acid or ester of itaconic acid is produced.

Description

FIELD OF THE INVENTION[0001]The present invention relates to a method for the production of itaconic acid and / or itaconate methylester by fermentation. The invention further relates to a fermentation broth comprising itaconic acid and / or itaconate methylester obtainable by such a process.BACKGROUND TO THE INVENTION[0002]Itaconic acid, an essential precursor to various products (e.g., acrylic fibers, rubbers, artificial diamonds, and lens), is in high demand in the chemical industry. Conventionally, itaconic acid is isolated from the filamentous fungus Aspergillus terreus. In addition, itaconic acid esters may be key intermediates for both commodity and specialty chemicals. The itaconic acid mono-methyl esters, i.e. 4-methyl itaconate and 1-methyl itaconate are particularly interesting in this respect.[0003]Recently, Aspergillus niger has been genetically modified to produce itaconic acid (WO2009014437, WO2009104958) by overexpressing cis-aconitate decarboxylase (CAD) and / or a putati...

Claims

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Application Information

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IPC IPC(8): C12P7/44C12P7/62
CPCC12P7/44C12P7/62C12P7/46
Inventor ZHAO, ZHENG
Owner DSM IP ASSETS BV
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