Secretome profile-facilitated in vitro fertilization

Abstract

Secretome profiling improves the pregnancy success rate of in vitro fertilization processes, while reducing the risk of multiple births.

Description

RELATED APPLICATIONS[0001]This application is a continuation of U.S. patent application Ser. No. 15 / 856,266, filed Dec. 28, 2017, which is a divisional of U.S. patent application Ser. No. 15 / 648,296, filed Jul. 12, 2017, now U.S. Pat. No. 9,874,572, which is a divisional of U.S. patent application Ser. No. 15 / 052,475, filed Feb. 24, 2016, now U.S. Pat. No. 9,714,950, which is a continuation of U.S. patent application Ser. No. 13 / 081,463, filed Apr. 6, 2011, now Abandoned, which claims benefit of priority to U.S. Provisional Patent Application No. 61 / 321,448, filed Apr. 6, 2010, each of which is incorporated by reference to the same extent as though fully replicated herein.SEQUENCE LISTING[0002]This application includes a Sequence Listing, as set forth in an ASCII-compliant text file named “CCRMProtein_ST25.txt”, created on Apr. 6, 2011, and containing 2263 kilobytes, which is incorporated by reference to the same extent as though fully replicated herein.BACKGROUNDField[0003]The inve...

AI Technical Summary

Benefits of technology

[0013]The CDC data from 2006 shows that use of donor eggs in a 40 year old woman will result in a live birth 54% of the time. This compares to 20.6% using the woman's own eggs. Thus, an opportunity exists for the instrumentalities of this disclosure to facilitate success rates approximating the use of donor eggs when using one's own eggs, and may even permit higher success rates due to better selection of viable embryos.

[0014]In an embodiment, a system is provided for enhancing the pregnancy success rate of in vitro fertilization. The system includes an electronic system to configured to gather secretome data as a secretome profile of an embryo by quantitating proteins implicated in implantation success. A model is provided for use in recommending whether to implant the embryo on the basis of this secretome profile. The secretome date may be, for example, provided by use of mass spectroscopy or ELISA measurements.

Problems solved by technology

Due to expensive procedural costs, IVF is only attempted after the failure of less expensive fertility treatments.

Mammalian embryo implantation is a complex and intricate process involving numerous biological changes at both the embryo and endometrial level.

A blastocyst failing to implant or an endometrium failing to sustain growth and differentiation will result in spontaneous abortion.

Blastocyst stage transfers often result in higher pregnancy rates.

Despite progressively improving IVF pregnancy rates, the majority of transferred human embryos result in implantation failure.

Moreover, implantation success rates may decrease with the increasing maternal age, if donor eggs are not used.

However, embryo selection based on morphological qualities is not precise.

The transfer of multiple embryos, however, often results in multiple pregnancies, a major complication of IVF.

In general, multiple pregnancies, specifically, more than twins, hold maternal and fetal risks.

For example, multiple births are associated with increased risk of pregnancy loss, neonatal morbidity, obstetrical complications, and prematurity with potential for long term damage.

However, these limitations are not universally followed or accepted.

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