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Highly parallel assays for simultaneous identification of antibody sequences and binding partners

a technology which is applied in the field of high parallel assays for simultaneous identification of antibody sequences and binding partners, can solve the problems of not employing the wash step

Pending Publication Date: 2021-09-02
THE UNIV OF BRITISH COLUMBIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent describes a method for determining the sequence of an antibody and its binding partner, such as an autoantigen. The method involves expanding a sample of antibodies and distributing them into containers for further analysis. The containers can be microfluidic devices or microwell plates. The antibodies are captured on a solid substrate and exposed to an antigen display library for interaction with display particles. The captured antibodies are then sequenced using a variety of methods. The results of the sequencing reactions identify the antibody and its binding partner. The method can be used to diagnose autoimmune disorders or to evaluate the immune response to transplantation.

Problems solved by technology

However, in another embodiment, a wash step is not employed.
However, in another embodiment, a wash step is not employed.

Method used

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  • Highly parallel assays for simultaneous identification of antibody sequences and binding partners
  • Highly parallel assays for simultaneous identification of antibody sequences and binding partners
  • Highly parallel assays for simultaneous identification of antibody sequences and binding partners

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Embodiment Construction

[0016]As used herein, “an antibody” is a polypeptide belonging to the immunoglobulin superfamily. In particular, “an antibody” includes an immunoglobulin molecule or an immunologically active fragment of an immunoglobulin molecule (i.e., a molecule(s) that contains an antigen binding site), an immunoglobulin heavy chain (alpha (α), mu (μ), delta (δ) or epsilon (ε)) or a variable domain thereof (VH domain), an immunoglobulin light chain (kappa (κ) or lambda (λ)) or a variable domain thereof (VL domain), or a polynucleotide encoding an immunoglobulin molecule or an immunologically active fragment of the immunoglobulin molecule. The antibody includes a single chain antibody (e.g., an immunoglobulin light chain or an immunoglobulin heavy chain), a single-domain antibody, an antibody variable fragment (Fv), a single-chain variable fragment (scFv), an scFv-zipper, an scFv-Fc, a disulfide-linked Fv (sdFv), a Fab fragment (e.g., CLVL or CHVH), a F(ab′) fragment, an epitope-binding fragment ...

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Abstract

The present invention relates to methods for isolating the sequences of an antibody that reacts with a disease related antigen, e.g., an autoantigen, without knowing the identity of the antigen (sequence or structural epitope) a priori. The methods can also be used to identify an antigen that mediates a disease state, e.g., an autoantigen implicated in an autoimmune disorder or a tumor response.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation application of U.S. application Ser. No. 16 / 063,394, filed Jun. 18, 2018; which is a national phase application corresponding to International Application No. PCT / CA2016 / 000323, filed Dec. 21, 2016; which claims priority from U.S. Provisional Application Ser. No. 62 / 270,094, filed Dec. 21, 2015, the contents of each of which are incorporated herein by reference in their entirety for all purposes.BACKGROUND OF THE INVENTION[0002]The adaptive immune response produces a diversity of different antibodies (on the order of 1011 unique sequences in a single individual), each of which is capable of binding to a different target epitope. The isolation of antibodies that bind to a specific target, let alone a specific epitope on a known target, is of high interest for the development of new products in therapeutics, diagnostics, and more generally, for research purposes. Methods for identifying such antibodies hav...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/10C12Q1/6874G01N33/566G01N33/68
CPCC12N15/1037G01N33/6854G01N33/566C12Q1/6874C12Q1/68C12N15/1034C40B40/02C40B50/06G01N33/6845
Inventor FALCONER, ESTERHANSEN, CARL LARS GENGHISLECAULT, VERONIQUELISAINGO, KATHLEENHEYRIES, KEVIN ALBERT
Owner THE UNIV OF BRITISH COLUMBIA