The invention discloses a preparation method for
antigen liquid for diagnosing
pulmonary acariasis by adopting the
skin pricking method. The preparation method comprises the following steps: firstly, collecting flour contaminated with dust from a flour mill; secondly, inspecting and separating mites through a
microscope and feeding the mites; thirdly, when the density of the mites reaches 2000 per
gram of feed base, positioning the mites into a
sieve to remove the impurities; fourthly, positioning the
powder sieved in the third step into a flat bottom enamel disk and separating the
powder mites; fifthly, positioning live
powder mites into a nylon bag, and immersing the mites into
acetone for
degreasing; sixthly, after washing, positioning the powder mites on the surface of a dish uniformly, then positioning the mites into a warm box to obtain dried powder mites; seventhly, immersing the dried powder mites into 9 percent of
sodium chloride, and positioning the mites into the environment at 4 DEG C; eighthly, taking out the
sodium chloride liquid in the seventh step, mixing the obtained suspension liquid, and taking supernatant through
centrifugation; and ninthly, filtering the supernatant for removing
bacteria, and obtaining the
antigen liquid of the powder mites. The
antigen liquid greatly prolongs the storage time, causes no pain to the patient and small
skin irritability when being used for diagnosing
pulmonary acariasis, is widely applicable in various medical institutions, and provides good basis for the diagnosis of the
pulmonary acariasis.