The invention provides a soil gas stratified sampler and a sampling and well washing method. The sampler comprises a sampling mechanism, well washing mechanism, a filter mechanism and a medium conveying hose, wherein the sampling mechanism is composed of a sampling main body, a sampling probe rod, a sampling probe, a plurality of connecting mechanisms and extension rods of which the number is twice that of the connecting mechanisms, the sampling mechanism is used for performing stratified sampling of soil and determining the optimal sampling time, the well washing mechanism is used for injecting a well washing medium from a sampling device, a medium in a shaft is carried to the ground, so that the process that the medium property in the wellbore meets the operation requirement is changed,the filter mechanism is arranged on one side in the sampling main body and is used for filtering the medium obtained by sampling, the filtered medium is conveyed into an analyzer to be analyzed and treated, the medium conveying hose is arranged between the sampling mechanism and the filter mechanism and used for conveying the obtained medium. The sampler is advantaged in that the sampler can be used for rapidly detecting concentrations of various gases in soil in a layered manner and can be used for carrying out water removal and dust removal on the gases, so subsequent analysis on the sampledgases is facilitated.