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Molecular mark of wild mountain ginseng and discrimination method therefor

A technology of wild ginseng and ginseng, which is applied in the field of medicine, can solve the problems of instability, small sample size, and lack of practicability, and achieve the effect of high stability and strong reliability

Inactive Publication Date: 2008-05-28
FUDAN UNIV +1
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Problems solved by technology

Most of the researchers did not obtain the unique molecular markers of wild ginseng (Ma Xiaojun et al., Research on the RAPD fingerprint of wild ginseng. Acta Pharmacica Sinica, 1999, 34(4): 312-316), and a few researchers obtained some of the unique molecular markers of wild ginseng, However, on the one hand, the amount of samples is too small, and on the other hand, it is unstable, so it lacks practicability (Ding Jianmi, Wan Shuwen, Mei Qichun. Yeshan participates in the identification technology research of Yishan ginseng and cultivated ginseng. Shanghai Journal of Preventive Medicine, 2001, 13(8): 359-371)

Method used

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  • Molecular mark of wild mountain ginseng and discrimination method therefor
  • Molecular mark of wild mountain ginseng and discrimination method therefor
  • Molecular mark of wild mountain ginseng and discrimination method therefor

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Embodiment Construction

[0047] There are 15 ginseng samples (L~Z), among which sample Z was collected from Tonghua, Jilin, and was dug from the forest by local farmers, and samples L~X were purchased from the market (only ginseng fibrous roots), and they were all called wild ginseng. name. The operation steps of the detection are as described above. Get 0.005g of fibrous root of each sample, carry out DNA extraction, and detect and confirm; Identify with ginseng primer (Seq.No.4+Seq.No.5) (results see Figure 7) , all have unique fragments of ginseng, therefore, all samples are ginseng. Use primers (Seq.No.2+Seq.No.3) to identify (results see Figure 8) , only samples S, Y and Z were different from cultivated ginseng and wild ginseng. Mix two pairs of primers (Seq.No.2+Seq.No.3, Seq.No.4+Seq.No.5) to detect the same sample, and the result is consistent with the above two detection results (see Figure 9) . During the detection process, the PCR reaction conditions are as follows:

[0048] (1)94℃ 9...

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Abstract

The present invention is the identifying method for wild mountain ginseng. The present invention obtains ginseng specific DNA segment Seq No. 1, and designs one pair of specific primers Seq No. 2 and Seq No. 3. During detection, small ginseng sample is used in extracting DNA, and PCR proliferation with the specific primers is performed to judge whether the sample is wild mountain ginseng, cultivated ginseng or not ginseng. The method of the present invention uses little material, and has no damage to ginseng sample, high reaction stability and high identification reliability.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to molecular markers and identification methods of wild ginseng. Background technique [0002] Ginseng (Panax ginseng C.A.Myer) is an Araliaceae plant, and its root is one of the most valuable Chinese medicinal materials in my country. Ginseng is generally classified into three types: wild ginseng, immigrated ginseng, and cultivated ginseng. The quality and efficacy of wild ginseng and other ginseng vary greatly, and the market price can vary several times, dozens of times or even hundreds of times. Therefore, it is very important to identify the authenticity of wild ginseng. However, different types of ginseng are very similar in appearance, and it is difficult for ordinary people to distinguish them and are easily confused. There are a lot of ginseng in the market, and it is not uncommon to see shoddy ginseng. [0003] The traditional identification of ginseng main...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 张文驹万树文程舟陈家宽丁建弥王琼
Owner FUDAN UNIV
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