Method for detecting oligo-xylose in oligo-xylose tablets
A technology of xylo-oligosaccharide and detection method, applied in the field of detection, can solve the problems such as public reports and regulations without xylo-oligosaccharide detection, and achieve the effect of strong specificity
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[0045] Instruments, reagents and test samples
[0046] Instrument: High-pressure liquid phase system: 515 high-pressure pump, 7725 injection valve, column thermostat, 2414 differential refractive index detector, chromatographic workstation, vacuum pump or hair dryer.
[0047] Reagent: 4mol / L sulfuric acid: dilute 98% sulfuric acid with water, and calibrate the concentration.
[0048] 40% sodium hydroxide: take 40.0g sodium hydroxide, add 100ml water to dissolve it.
[0049] 60% ethanol
[0050] Absolute ethanol (AR)
[0051] Acetyl (chromatographic grade)
[0052] Xylose Standard
[0053] Test sample: xylooligosaccharide tablets
[0054] Chromatographic conditions
[0055] Chromatographic column: bonded amino column (nova-pak250×3.9mmid steel, 4um)
[0056] Mobile phase: acetonitrile: water (75:25v / v)
[0057] Flow rate: 1.0ml / min
[0058] Column temperature: 35°C
[0059] Differential detector temperature: 35°C
[0060] Injection volume: 20μl
[0061] Test method ...
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