Combined application of artemisinin and its derivative and antibiotic medicine

A technology of antibacterial drugs and artesunate, applied in the direction of antibacterial drugs, medical preparations containing active ingredients, pharmaceutical formulas, etc., to reduce abuse and improve antibacterial efficacy

Inactive Publication Date: 2010-02-03
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether artesunate has antibacterial effect, whether it can improve the resistance of bacteria to antibiotics, whether it can enhance the antibacterial efficacy of antibiotics and whether it is effective against infections caused by bacteria has not been reported.

Method used

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  • Combined application of artemisinin and its derivative and antibiotic medicine
  • Combined application of artemisinin and its derivative and antibiotic medicine
  • Combined application of artemisinin and its derivative and antibiotic medicine

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0023] This experimental example is to study the MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) of artesunate and ten antibacterial drugs against different bacteria

[0024] Using the microwell dilution method, the bacterial concentration was adjusted to 105 CFU / ml, and inoculated in a 96-well sterile culture plate. Artesunate, penicillin sodium, ampicillin and other 10 antibacterial drugs were diluted with normal saline to 5.14 mg / ml. Add various drugs into the culture wells containing bacteria, and serially dilute, the final concentrations of drugs in wells 1 to 10 are 256, 128, 64, 32, 16, 8, 4, 2, 1, 0.5ug / ml. Incubate in a 37°C incubator for 24h and 48h, read the positive and negative control wells, the negative control wells are clear, and the positive control wells are turbid. The MIC of a drug on bacteria is the lowest drug concentration that inhibits the growth of bacteria visible to the naked eye after 24 hours, and the MBC of the...

experiment example 2

[0028] This experimental example is to study the effect of artesunate combined with antibacterial drugs on MIC and MBC of Escherichia coli.

[0029] Using the checkerboard microwell dilution method, the bacterial concentration was adjusted to 105 CFU / ml, inoculated in a 96-well sterile culture plate, and the antibacterial drugs that were moderately sensitive to five kinds of bacteria were mixed with different concentrations of artesunate at a dose lower than the MIC, respectively. Compatibility, observe the MIC and MBC of the compatible drugs against Escherichia coli. The results of this experiment show that although artesunate used alone cannot completely inhibit the growth of bacteria, it can significantly reduce the MIC and MBC of antibacterial drugs when used in combination with antibacterial drugs, indicating that artesunate and antibacterial drugs have a synergistic effect on bacteria. Bacteriostatic efficacy. (See Table 4).

[0030] Table 4 The minimum inhibitory conc...

experiment example 3

[0033] This experimental example is to study the inhibitory strength of artesunate and 1 / 2 MIC concentration of different antibacterial drugs on the growth of Escherichia coli.

[0034] Adjust the bacterial concentration to be 106CFU / ml, and measure the OD600 of the bacterial solution to be 0.002 (1OD=5×10 8 CFU / ml), referring to the results of Experimental Example 1, separately added antibacterial drugs with a final concentration of 256 μg / ml artesunate or 1 / 2 MIC concentration, and simultaneously added 256 μg / ml artesunate and 1 / 2 MIC concentration After antibacterial drugs, shake at 150rpm on a constant temperature shaker at 37°C, measure the OD value of the bacterial solution at 1, 3, 5, 7, 9, 12, 16, and 24 hours, and calculate the amount of bacteria at each time point. The results of the study showed that artesunate 256 μg / ml could significantly slow down the growth rate of Escherichia coli ATCC35218, Staphylococcus aureus ATCC25923 and clinical isolates of Escherichia c...

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Abstract

The present invention discloses the new use of available antimalarial arteannuin and its derivatives dihydro arteannuin, antiannuic methyl ether, antiannuic ethyl ether and antiannuic amber. Arteannuin and its derivatives are used through combination with antibiotic medicine to inhibit bacterial growth and enhance the antibiotic effect of available antibiotic medicine. Especially, antiannuic amberis applied in preventing and treating bacterial infection diseases except being used as antimalarial.

Description

technical field [0001] The invention relates to the field of medicine application, in particular to the antibacterial application of artemisinin and its derivatives. Background technique [0002] Infectious disease is a disease caused by pathogenic microorganisms invading the body and multiplying in large quantities in the body. It is one of the important reasons for the death of clinical patients. One of the causes of the patient's death is that bacteria proliferate and release toxins that cause damage to tissues and organs; the other is that bacterial components induce the release of inflammatory cytokines such as TNF-α, IL-1, IL-6, and NO by activating immune cells. The cascade effect formed by a variety of inflammatory mediators can cause the inflammatory response to expand or even get out of control, and eventually lead to the occurrence of systemic inflammation characterized by cell self-destruction, that is, sepsis. Bacterial infection was once the number one cause o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/06A61K31/357A61K31/43A61K31/496A61K31/545A61K31/7036A61K31/7056A61P31/04
Inventor 周红李斌郑江
Owner ARMY MEDICAL UNIV
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