Protein chip used for detecting functional injury of central nervous system and manufacturing method therefor

A technology of central nervous system and protein chip, applied in the biological field, can solve the problems of large amount of data and high cost, and achieve the effect of simple research work, accelerated speed and process.

Inactive Publication Date: 2007-08-01
陈云 +3
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional high-throughput gene expression profiling chips are costly and requir

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 (protein antibody chip):

[0040] a. Nerve injury-specific protein factor antibody:

[0041] Monoclonal antibodies to homologous protein factors (markers of nerve injury) of 381 specific genes closely related to central nervous system injury listed in Table 1 were used as central nervous system injury-specific protein factor antibodies.

[0042] b. Extraction of proteins in patient blood, cerebrospinal fluid or damaged tissue samples:

[0043] Use RIPA solution (1% Igepal CA-630, 0.5% Sodium deoxycholate, 0.1% SDS and PBS, add 10mMbeta-mercaptoethanol, 10ug / ml PMSF, 5ug / ml aprotinin for the extracted patient’s blood, cerebrospinal fluid or damaged tissue samples) , 0.1mg / ml benzamidine, 1ug / ml pepstatin A, 1ug / ml leupeptin, 100mM sodium orthovanadate and 15ul / ml Triton X-100) extract protein, homogenate and centrifuge and store at -70°C.

[0044] c. Fabrication of protein antibody chips and fixation of nerve injury-specific protein factor antibodies:

[...

Embodiment 2

[0052] Embodiment 2 (protein antigen chip)

[0053] a. Central nervous system injury-specific protein antigen:

[0054] The homologous protein-specific proteins (which can be ordered from other companies) of 381 genes closely related to central nervous system injury listed in Table 1 were used as central nervous system injury-specific protein antigens.

[0055] b. Patient serum or cerebrospinal fluid samples:

[0056] The patient's blood or cerebrospinal fluid samples were taken, centrifuged to obtain serum or cerebrospinal fluid supernatant, and stored at -70°C for later use.

[0057] c. Fabrication of protein antigen chips and fixation of nerve injury-specific protein antigens:

[0058] Using a high-speed automatic microarray spotting machine (CM-1000), 381 kinds of specific protein antigens closely related to central nervous system injury listed in Table 1, positive control protein antigens (Caspases, Tumor necrosis factor, etc.), negative control protein antigens ( Huma...

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PUM

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Abstract

This invention relates to one functional central neutral damage dialogue test protein chip and its process method, which comprises the following steps: using protein chip to fix multiple central neutral system damage generation development key function abnormal protein factor antigen and its array; the said protein antigen and its array abnormal protein factors act as key property protein factors in the neutral system for inflammation and activation reaction and cell ion balance adjust and signal transmission.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a protein chip for diagnosis and detection of functional central nervous system damage and a manufacturing method thereof, in particular to a specific detection and central nervous system damage (including brain damage caused by cerebral apoplexy or cerebrovascular accident) , Traumatic Brain Injury and Spinal Cord Injury) biochips of major neurotrauma-specific protein factors that are closely related to pathophysiological and molecular biological changes. Background technique [0002] Central nervous system injury (including brain injury caused by cerebral apoplexy or cerebrovascular accident, traumatic brain injury and spinal cord injury) is a major disease that threatens human health. It results in severe disability and an unusually high mortality rate, as well as enormous medical rehabilitation costs. Every year in the United States, about 430,000 people suffer from cerebral apopl...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/577
Inventor 陈云刘谦黄卫宫岩华
Owner 陈云
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