Tissue culture method for chrysanthemum under LED condition

Abstract

The invention provides a new method for tissue culturing chrysanthemum. It employs cold light source (light-emitting diode) as light source, and determines the optimum ratio of red light to blue light and the best light emitting strength. It takes poromeric fluoroethylene resin film as culturing container for tissue culture sprout; and employs rock fiber block as culture medium. The growing speed for tissue culture sprout is one time faster than that with normal culture method. The tissue culture sprout and replanting sprout are characterized by largest plant height, largest leaf number, root number, longest root length and largest dry net weight of haulm and root, high rate of emergence and little pollution rate.

Description

Technical field [0001] The invention relates to plant tissue culture technology, in particular to a chrysanthemum tissue culture method. Background technique [0002] Chrysanthemum (Dendranthema×grandflora Tzvel.) is one of the world’s four major cut flowers, accounting for 23% of the world’s flower output and flower sales. Chrysanthemum also occupies a large proportion in my country’s flower production and is one of my country’s traditional famous flowers. , Has a long history of cultivation and breeding. With the rapid development of biotechnology today, the tissue culture of chrysanthemum has greatly promoted the large-scale and factory production of chrysanthemum. [0003] In the process of plant tissue culture, environmental factors have a great influence on the growth and development of tissue cultured seedlings. Light is one of the most important environmental factors. The development of high-efficiency artificial light sources has always been a very important subject in t...

AI Technical Summary

Problems solved by technology

[0004] The traditional plant tissue culture method uses highly airtight containers to prevent pollution. The environment inside and outside the container is very different. The humidity in the culture container is high, the gas fluidity is poor, the carbon dioxide is insufficient, and the concentration of toxic substances in secondary metabolism is high. Serious microbial contamination

These can directly lead to changes in the morphology and physiology of the tissue culture seedlings, such as slow growth, easy glass seedlings, and poor adaptability to the external environment after emergence.