Protein hydrolysate with antidiabetic effect

An anti-diabetic, hydrolyzate technology, applied in the composition of action, the composition of diabetes and diabetic complications, the prevention or treatment of diseases caused by hyperglycemia, can solve the effective treatment or prevention of diabetes and its complications without Completely build etc.

Active Publication Date: 2007-10-24
MG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] It can therefore be said that effective methods of treating or preventing diabetes and its complications are not fully established

Method used

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  • Protein hydrolysate with antidiabetic effect
  • Protein hydrolysate with antidiabetic effect
  • Protein hydrolysate with antidiabetic effect

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0071] Preparation Example 1 Preparation of globulin hydrolyzate

[0072] A method for preparing a globulin hydrolyzate using bovine erythrocytes is described in detail below.

[0073] Add 250 liters of water to 100 kg of fresh bovine erythrocytes for sufficient hemolysis. After adjusting the pH to 2.8 with phosphoric acid, the 2.6×10 7 A unit of acid protease from Aspergillus niger was added to the solution, and reacted at 50° C. for 3 hours.

[0074] After the reaction, the reaction solution was heated at 80° C. for 30 minutes to terminate the reaction. Thereafter, a suspended aqueous solution of calcium hydroxide was added to the reaction solution to adjust the pH to 6.5. Then, 10 kg of diatomaceous earth was added, and the mixture was filtered with a filter press. The resulting filtrate was spray dried to yield 23 kg of powdered globulin hydrolyzate. The molecular weight distribution of the globulin hydrolyzate was examined by gel filtration chromatography performed u...

preparation Embodiment 2

[0082] Preparation Example 2 Fraction and Purification of the Peptide Suppressing Blood TG Level Elevation

[0083] The peptides of the present invention are obtained by (1) ion exchange, (2) ultrafiltration, (3) separation by reverse phase column chromatography under acidic conditions, and (4) separation by reverse phase chromatography under neutral conditions.

[0084] (1) Ion exchange

[0085] The 10% aqueous solution containing the 13.7g globulin hydrolyzate obtained in Preparation Example 1 was added to weakly acidic cation exchange resin (Amberlite IRC 50 , H + Type, Organo Co., Ltd.), stirring for 1 hour to absorb. The unabsorbed fraction is thus separated off.

[0086] (2) ultrafiltration

[0087] The unabsorbed fraction obtained by the aforementioned ion exchange was subjected to ultrafiltration using an agitation force ultrafiltration device (manufactured by Advantec, UHP 90K) and an ultrafiltration membrane (manufactured by Advantec, UIIH-1, molecular weight cut...

Embodiment 1

[0160] Embodiment 1 prepares the food containing peptide (VVYP)

[0161] (1) Preparation of milk powder

[0162] 10 milligrams of peptides prepared in Preparation Example 2 with the amino acid sequence of Val-Val-Tyr-Pro (SEQ.ID.No.1) added to 100 g of infant milk formula to obtain a peptide with the ability to promote insulin secretion or inhibit blood sugar elevation Functional milk powder.

[0163] (2) Preparation of chocolate

[0164] Add 50 mg of the peptide prepared in Preparation Example 2 with the amino acid sequence of Val-Val-Tyr-Pro (SEQ.ID.No.1) to 100 g of chocolate, so as to obtain the peptide with the function of promoting insulin secretion or inhibiting blood sugar rise. chocolate.

[0165] (3) Preparation of green tea beverage

[0166] 80 kg of green tea leaves were added to 300 l of hot water (80° C.) and extracted at this temperature for 4 minutes. The resulting extract was cooled and centrifuged. The clear supernatant was collected as green tea extrac...

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Abstract

The present invention provides compositions (blood sugar increase inhibitors) that have an action of lowering the blood sugar level of a subject in a hyperglycemic condition and are for use in lowering the blood sugar level of such a subject. Moreover, the present invention provides compositions for use in preventing or treating diseases resulting from hyperglycemia, in particular, diabetes and diabetic complications, due to the aforementioned action (a preventive or ameliorative composition for diseases resulting from hyperglycemia, an antidiabetic agent).

Description

technical field [0001] The present invention relates to insulin sensitivity exhibited by inducing insulin secretion or by increasing insulin receptors in diabetic subjects or in preclinical (borderline diabetic) subjects to prevent or alleviate hyperglycemic states (suppression of blood sugar) Elevated effect) composition (hyperglycemia inhibitor, insulin secretion enhancer). Furthermore, the present invention relates to compositions for preventing or treating diseases caused by hyperglycemia, especially diabetes and diabetic complications. In this specification, these compositions are sometimes collectively referred to as antidiabetic compositions. [0002] Furthermore, the present invention relates to a method for lowering the blood sugar level of a subject in a state of hyperglycemia, and also relates to the prevention or treatment of diseases caused by hyperglycemia (specifically, diabetes and Complications of diabetes). Background technique [0003] In the living bod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/00A61P3/10A23L1/305C07K5/103C07K14/47
CPCA23V2002/00C07K14/47C07K5/101A23L1/3053C07K1/12A61K38/017A61K38/07A23L33/18A61P1/04A61P21/00A61P25/00A61P27/02A61P3/08A61P5/50A61P9/00A61P3/10A23V2200/328A23V2250/542A61K38/16
Inventor 香川恭一成瀬年彦福浜千津子
Owner MG PHARMA
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