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Process of preparing citric acid with side product sugar liquid from peltate yam sapanin production

A technology of by-product sugar and citric acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of increased burden, low value of fermentation broth filter residue, fluctuation of nutrient content of medium, etc., and achieve low cost Effect

Inactive Publication Date: 2010-06-23
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former is still crude material fermentation, which brings a series of inherent defects, such as fluctuations in the nutrient composition of the medium; a large number of impurities that do not participate in biochemical reactions waste energy and add a lot of burden to the extraction; the total sugar concentration of the fermentation liquid is low, It affects the fermentation index and equipment utilization; the value of the filter residue of the fermentation broth is very low, and it is difficult to comprehensively utilize it, etc.

Method used

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  • Process of preparing citric acid with side product sugar liquid from peltate yam sapanin production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Such as figure 1 Shown, utilize the method for preparing citric acid from the by-product sugar liquid of turmeric to produce saponin, it comprises the steps:

[0020] 1) Adjust the reducing sugar volume concentration of the by-product sugar solution produced by turmeric saponin to 10% (i.e. pretreatment, deployment), after being clarified by a clarifier, sterilized by steam, and then put into a fermentation tank (such as a seed fermentation tank) , commercial fermentation tank) as a nutrient carbon source, adding dipotassium hydrogen phosphate, ammonium sulfate and MgSO 4 ·7H 2 0, get the by-product sugar liquid culture medium; Wherein, by the by-product sugar liquid of producing saponin by turmeric: dipotassium hydrogen phosphate=100 milliliters: 0.05 gram adds dipotassium hydrogen phosphate, by the by-product sugar liquid of producing saponin by turmeric: Ammonium sulfate=100 milliliters: 0.2 grams add ammonium sulfate, produce saponin by-product sugar liquid accord...

Embodiment 2

[0026] Utilize the method for preparing citric acid from the by-product sugar liquid of turmeric to produce saponin, it comprises the steps:

[0027] 1) Adjust the reducing sugar volume concentration of the by-product sugar liquid produced by turmeric saponin to 14%, after being clarified by a clarifier, sterilize by steam, then put it into a fermentation tank as a nutritional carbon source, add dipotassium hydrogen phosphate, Ammonium Sulfate and MgSO 4 ·7H 2 0, get the by-product sugar liquid culture medium; Wherein, by the by-product sugar liquid of producing saponin by turmeric: dipotassium hydrogen phosphate=100 milliliters: 0.1 gram adds dipotassium hydrogen phosphate, by the by-product sugar liquid of producing saponin by turmeric: Ammonium sulfate=100 milliliters: 0.4 grams add ammonium sulfate, produce saponin by-product sugar liquid according to turmeric: MgSO 4 ·7H 2 O=100ml: 0.2g MgSO added 4 ·7H 2 O;

[0028] 2) control figure 1 , the Aspergillus niger prog...

Embodiment 3

[0032] Utilize the method for preparing citric acid from the by-product sugar liquid of turmeric to produce saponin, it comprises the steps:

[0033] 1) Adjust the reducing sugar volume concentration of the by-product sugar solution produced by turmeric saponin to 20%, and the by-product sugar solution produced by turmeric saponin is clarified by a clarifier, sterilized by steam, and then put into a fermentation tank as nutrition Carbon source, add potassium dihydrogen phosphate, ammonium sulfate and MgSO 4 ·7H 2 0, get the by-product sugar liquid culture medium; Wherein, by the by-product sugar liquid of producing saponin by turmeric: potassium dihydrogen phosphate=100 milliliters: 0.15 gram adds potassium dihydrogen phosphate, by the by-product sugar liquid of producing saponin by turmeric: Ammonium sulfate=100 milliliters: 0.8 grams add ammonium sulfate, produce saponin by-product sugar liquid according to turmeric: MgSO 4 ·7H 2 O=100ml: 0.4g MgSO added 4 ·7H 2 O;

[...

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Abstract

The present invention relates to is process of preparing citric acid with side product sugar liquid from peltate yam saponin production. The process includes the following steps: 1. clarifying the sugar liquid in a clarifying machine, steam sterilizing, setting in fermenting tank for serving as the nutritious carbon source, adding dipotassium hydrogen phosphate or potassium dihydrogen phosphate, adding ammonium nitrate or ammonium sulfate, adding MgSO4.7H2O to obtain sugar liquid culture medium; 2. activating Aspergillus niger to prepare spore suspension; 3. inoculating Aspergillus niger to the sugar liquid culture medium in the volume ratio between the Aspergillus niger spore suspension and the culture medium of 1 to 10-50, and culturing at 30-35 deg.c for 70-100 hr to obtain the fermented liquid; and 4. centrifugally separating the fermented liquid, washing and precipitating to obtain citric acid. The present invention features low cost and environment friendship.

Description

technical field [0001] The invention relates to a method for preparing citric acid from the by-product sugar solution produced by turmeric to produce saponin. Background technique [0002] Citric acid, also known as citrate, exists in natural fruits. In 1968, our country obtained the success of submerged fermentation of citric acid in dried potato raw materials. Submerged fermentation of dried potato raw materials produces citric acid. The raw materials are abundant, the mine process is simple, and the fermentation level is high. There are factories producing citric acid in many places. Citric acid is a five-color transparent crystal or white powder with an attractive sour taste. It is an important raw material for food, chemical, pharmaceutical, and textile industries. [0003] The strain used to produce citric acid is Aspergillus niger. Aspergillus niger not only can utilize starch, but also has a certain ability to decompose protein, cellulose, and pectin substances. I...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/48C12R1/685
Inventor 刘慧王焰新鲍建国
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)