Low-temperature lipase and its gene sequence

Abstract

The invention discloses a low- temperature lipase and its code gene and application. The gene of low- temperature lipase is 70% homologous to DNA squence according to SEQ No. 1 limit in sequence table, and is DNA squence of functinoal protein with same code. The protein of low- temperature lipase code is protein with SEQ No. 2 amnio acid squence in squence table or protein with same activity derived from squence 2. The invention aslo discloses a method for constructing low- temperature lipase engineering bacteria and method for preparing low- temperature lipase by using said engineering bacteria. The activity and stability of low- temperature lipase is pretty goos at low temeprature, and can be used in fields of washing agent industry, foodstuff industry, biological pharmacy and environmental biological technique. The application prospect is wide.

Description

technical field

[0001] The invention relates to a low-temperature lipase isolated and purified from Pseudomonas sp and its coded gene sequence, the construction of a low-temperature lipase engineering bacterium, and a method for preparing the low-temperature lipase by using the engineering bacterium. Background technique

[0002] Lipase (lipase, EC3.1.1.3), also known as glyceride hydrolase, refers to an enzyme that decomposes triglyceride ester bonds formed by higher fatty acids and glycerol, and is widely found in animal tissues, plant seeds and microorganisms.

[0003] As an important industrial enzyme, lipase can be used in oil hydrolysis, improvement of food flavor and aroma, medical medicine, degreasing of leather and silk spinning, modification of low-grade oil, addition to detergents and cosmetics, etc. Especially in the oleochemical and organic synthesis industries, enzyme-catalyzed reactions have the advantages of mild conditions, low energy consumption, low raw ma...

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