Polyglucose and its extracting method and application

A technology for grape polysaccharide and grapes is applied in the field of extracting grape polysaccharide, which can solve the problems of deepening the color of the final product and breaking the polysaccharide, and achieve the effect of simple extraction method and good application prospect.

Inactive Publication Date: 2010-12-22
XINJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the previous polysaccharide extraction process, there were many long-term high-heat extraction processes, such as decoction and concentration, which would not only deepen the color of the final product, but also break many polysaccharides into oligosaccharides and monosaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1. Preparation of grape polysaccharides in fresh grapes:

[0016] Take 5000 grams of fresh grapes, after sorting, rinse with tap water, press the juice, remove the grape skins and grape seeds, filter, and concentrate the filtrate under reduced pressure. Add 4 times or 9 times the amount of 95% ethanol to the concentrated solution, stir well at room temperature, let stand for 4 hours or 12 hours for precipitation, filter, recover the ethanol, collect the crude polysaccharides, wash the filter cloth with water 3 times, and wash the liquid with the crude The polysaccharides are combined, stirred and dissolved to obtain a crude polysaccharide aqueous solution, filtered, and the filtrate is collected. After the filtrate is passed through an ultrafiltration membrane and a reverse osmosis membrane, the grape polysaccharide concentrate is obtained. .

Embodiment 2

[0017] Example 2. Preparation of grape polysaccharides in raisins:

[0018] Take 2000 grams of raisins, after sorting, rinse them with tap water, dry them at room temperature, put them in a container, add 10 times the amount of distilled water, extract 3 hours at 80°C for 3 times, combine the extracts, and concentrate at 50°C under reduced pressure. Add 4 times or 9 times the amount of 95% ethanol to the concentrated solution, stir well at room temperature, let stand for 4 hours or 12 hours for precipitation, filter, recover the ethanol, collect the crude polysaccharides, wash the filter cloth with water 3 times, and wash the liquid with the crude The polysaccharides are combined, stirred and dissolved to obtain a crude polysaccharide aqueous solution, filtered, and collected the filtrate. After passing the filtrate through an ultrafiltration membrane and a reverse osmosis membrane, a grape polysaccharide concentrate is obtained. The grape polysaccharide concentrate can be used di...

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PUM

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Abstract

The invention relates to the technical field for extracting vitis linn polysaccharide from grapes or raisins, and provides a method of extracting and the applications of the vitis linn polysaccharide. The extraction steps are as follow: ethanol with the concentration of 95 percent whose weight is 4 to 9 times as of grape concentrated liquid is put into the grape concentrated liquid, and then the solution is stirred fully in room temperature and keeps standing for 4 to 12 hours to precipitate crud polysaccharide which is filtered by a piece of filter cloth with 300 meshes to 500 meshes to collect the crude polysaccharide. The invention can extract the vitis linn polysaccharide effectively with simple method, the vitis linn polysaccharide can be applied to prepare drug combinations for treating diseases caused by abnormal immune functions or for regulating abnormal immune functions caused by the disease. The vitis linn polysaccharide has uniform and strong protective immune function in vivo and vitro studies, and has a good application prospect as an immunopotentiator and an immunomodulator.

Description

Technical field [0001] The invention relates to the technical field of extracting grape polysaccharides from grapes or raisins, and relates to a grape polysaccharide and an extraction method and application thereof. Background technique [0002] Grape is a commonly used plant for medicine and food in my country’s traditional medicine and Uyghur medicine. Polysaccharides are one of the main active ingredients in grapes. Its main effect is to improve the body’s immune ability, thereby curing diseases caused by abnormal immune function, such as Colds, measles, and hepatitis. The sugars in grapes are mostly monosaccharides and oligosaccharides, and the polysaccharide content is greater than 6%, which is relatively small. In the past polysaccharide extraction process, there are usually long-time high-heat extraction processes, such as decoction and concentration, which will not only deepen the color of the final product, but also break many polysaccharides into oligosaccharides and mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P37/00
Inventor 马龙刘涛赵军周晓辉
Owner XINJIANG MEDICAL UNIV
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