Methods of treating ear infections
A technology of otitis media, antimicrobial agent, applied in the field of ear infection treatment, which can solve the problem without causing systemic effects
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Embodiment 1-4
[0380] Antimicrobial compositions were prepared using the components shown in Table 1. The components were combined and heated to give a clear solution that remained clear on cooling to room temperature. These solutions were used for further antimicrobial testing.
[0381]
[0382] NA - not applicable
[0383] *These components can also function as penetrants.
Embodiment 5
[0384] Example 5 - Testing MIC using Streptococcus pneumoniae type 3
[0385] Minimum inhibitory concentrations were determined using agar dilutions according to procedures such as those described in National Committee for Clinical Laboratory Standards (1993); Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically-Third Edition: Approved Standard M1-A3. NCCLS, Villanova, PA (MIC). Decade dilutions from 1 / 10 to 1 / 10,000 were prepared for all compounds. The compositions of Examples 1-4 had MICs in the 1 / 1000 dilution range.
[0386] A second experiment was performed starting from a 1 / 100 dilution of each compound and measuring CFU / mL of the different dilutions. The results of those tests for 1 / 100, 1 / 500, 1 / 1000, and 1 / 5000 dilutions of each example solution are provided in Tables 2a-d below. The initial inoculation was 1×10 6 / mL organism. The samples were incubated at 37° C. in an incubator with 7% carbon dioxide and fixed. The concent...
Embodiment 6
[0392] Example 6. MIC test with Haemophilus influenzae
[0393] Bacterial kill was determined as described in Example 5, however, the organism used was atypical Haemophilus influenzae in Todd Hewitt's medium supplemented with NAD and Hemin. Example formulations 1, 2, and 4 were tested and the results are provided in Tables 3a-c below.
[0394]
[0395]
[0396]
[0397] Tables 3a-c show example formulations effective in killing atypical H. influenzae.
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