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A strain of hybridoma capable of stably excreting anti-URG11 monoclonal antibody

A monoclonal antibody, hybridoma cell technology, applied in the field of hybridoma cells, can solve problems such as unsatisfactory clinical effects

Inactive Publication Date: 2010-12-29
NO 1 AUXILARY HOSPITAL NO 4 MILLITARY MEDICAL UNIV P L A
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Existing studies preliminarily show that URG11 may be an oncogene, and may become a target gene for cancer treatment. Although many target genes for cancer treatment have been discovered, the clinical effects are not satisfactory. Further research is needed to discover more or clinical targets. Target gene for better cancer treatment

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  • A strain of hybridoma capable of stably excreting anti-URG11 monoclonal antibody
  • A strain of hybridoma capable of stably excreting anti-URG11 monoclonal antibody
  • A strain of hybridoma capable of stably excreting anti-URG11 monoclonal antibody

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Embodiment Construction

[0013] 1. Antigen: According to published literature (Lian Z, Liu J, Li L, Li X, TufanNL, Clayton M, Wu MC, Wang HY, Arbuthnot P, Kew M, and FeitelsonMA: Upregulated expression of a unique gene by hepatitis The polypeptide sequence (HPPDGSFSTFHKGPQPLEDPC) in B_antigen promotes hepatocellular growth and tumorigenesis.Neoplasia2003; 5:229-244.) is artificially synthesized as shown in Figure 1, and the polypeptide is linked to hemocyanin (peptide-KLH) or bovine Serum protein (peptide-BSA), and the peptide linked to KLH as the antigen.

[0014] 2. Immunization: BALB / C mice (4-8 weeks old, female) were immunized with KLH-linked polypeptides.

[0015] The first immunization: take 0.5ml peptide-KLH (concentration: 240ug / ml) and mix it with an equal volume of Freund's complete adjuvant, and inject BALB / C mice subcutaneously at multiple points;

[0016] The second immunization: After 3 weeks, take 0.5ml peptide-KLH (concentration: 240ug / ml) and mix it with an equal volume of Freund's ...

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Abstract

The present invention mainly relates to the genetic engineering, in particular to a hybridoma cell (3D2) which can stabilize a secrete anti-URG11 monoclonal antibody, and is a hybridoma cell line with the collection number of CGMCCNO.1908. The hybridoma cell line with the collection number of CGMCCNO.1908 is used for a secrete anti-URG11 monoclonal antibody hybridoma cell line. The present invention can stabilize the secrete anti-URG11 monoclonal antibody hybridoma cell line (3D2), further research the functions of URG11, thereby being used for the research of the cancer treatment.

Description

technical field [0001] The invention mainly relates to genetic engineering, especially a strain of hybridoma cell (3D2) capable of stably secreting anti-URG11 monoclonal antibody. Background technique [0002] Current research shows that URG11 is a new gene cloned by suppression subtractive hybridization and full-length PCR amplification (RACE-PCR) that can be up-regulated by HBx protein. exons, encoding a protein of 673 amino acids (Fig. 1) with a molecular weight of 70KD. [0003] Compared with the NIH Human Genome Database, this gene is located on human chromosome 11 ( figure 2 ), there is no homologous gene in the gene pool, and it is a new gene. Analysis of protein comprehensive information library (SWISSPROT): ①has a transmembrane helix (Transmembrane helices: 281-297); ②discovered two vascular adhesion molecule domains (VWC Domain, Von Willebrand factortype C), a plant lectin domain (Lectin Domain), it is speculated that this molecule may be related to the movement...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/12C12N5/18C07K16/18
Inventor 刘杰
Owner NO 1 AUXILARY HOSPITAL NO 4 MILLITARY MEDICAL UNIV P L A
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