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Hybridoma cell for resisting URG11 monoclonal antibody

A monoclonal antibody, hybridoma cell technology, applied in the field of genetic engineering and hybridoma cells, can solve problems such as unsatisfactory clinical effects

Inactive Publication Date: 2010-06-16
NO 1 AUXILARY HOSPITAL NO 4 MILLITARY MEDICAL UNIV P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing studies preliminarily show that URG11 may be an oncogene, and may become a target gene for cancer treatment. Although many target genes for cancer treatment have been discovered, the clinical effects are not satisfactory. Further research is needed to discover more or clinical targets. Target gene for better cancer treatment

Method used

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  • Hybridoma cell for resisting URG11 monoclonal antibody
  • Hybridoma cell for resisting URG11 monoclonal antibody
  • Hybridoma cell for resisting URG11 monoclonal antibody

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Embodiment Construction

[0013] 1. Antigen: According to published literature (Lian Z, Liu J, Li L, Li X, TufanNL, Clayton M, Wu MC, Wang HY, Arbuthnot P, Kew M, and FeitelsonMA: Upregulated expression of a unique gene by hepatitis B_antigen promotes hepatocellular growth and tumorigenesis. Neoplasia 2003; 5: 229-244.) in the polypeptide sequence (HPPDGSFSTFHKGPQPLEDPC), through artificial synthesis of polypeptides such as figure 1 shown, and the polypeptide was linked to hemocyanin (peptide-KLH) or bovine serum albumin (peptide-BSA), and the polypeptide linked to KLH was used as an antigen.

[0014] 2. Immunization: BALB / C mice (4-8 weeks old, female) were immunized with KLH-linked polypeptides.

[0015] The first immunization: take 0.5ml peptide-KLH (concentration: 240ug / ml) and mix it with an equal volume of Freund's complete adjuvant, and inject BALB / C mice subcutaneously at multiple points;

[0016] The second immunization: After 3 weeks, take 0.5ml peptide-KLH (concentration: 240ug / ml) and mix ...

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Abstract

The invention mainly relates to the gene engineering, in particular to a hybridoma cell (3D2) for resisting a URG11 monoclonal antibody, which is a hybridoma cell strain with a preservation number of CGMCC NO.1907. The hybridoma cell strain with a preservation number of NO.1907 is used for secreting the hybridoma cell strain resisting the URG11 monoclonal antibody. The invention can stably secrete the hybridoma cell (3D2) for resisting the URG11 monoclonal antibody so as to further research the function of the URG11, and is used for the research of the cancer treatment.

Description

technical field [0001] The present invention mainly relates to genetic engineering, especially a strain of hybridoma cell (3D2) of anti-URG11 monoclonal antibody. Background technique [0002] Current research shows that URG11 is a new gene cloned by suppression subtractive hybridization and full-length PCR amplification (RACE-PCR) that can be up-regulated by HBx protein. exons, encoding 673 amino acids ( figure 1 ), a protein with a molecular weight of 70KD. [0003] Compared with the NIH Human Genome Database, this gene is located on human chromosome 11 ( figure 2 ), there is no homologous gene in the gene pool, and it is a new gene. Analysis of protein comprehensive information library (SWISSPROT): ①has a transmembrane helix (Transmembrane helices: 281-297); ②discovered two vascular adhesion molecule domains (VWC Domain, Von Willebrand factortype C), a plant lectin domain (Lectin Domain), it is speculated that this molecule may be related to the movement and metastas...

Claims

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Application Information

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IPC IPC(8): C12N5/20C07K16/18
Inventor 刘杰
Owner NO 1 AUXILARY HOSPITAL NO 4 MILLITARY MEDICAL UNIV P L A
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