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Hybridoma cell for resisting URG4 monoclonal antibody

A technology of hybridoma cells and hybridoma cell lines, applied in the field of hybridoma cells that can stably secrete anti-URG4 monoclonal antibody

Inactive Publication Date: 2010-06-16
NO 1 AUXILARY HOSPITAL NO 4 MILLITARY MEDICAL UNIV P L A
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, its specific mechanism of action in the formation of malignant tumors needs to be further studied to clarify

Method used

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  • Hybridoma cell for resisting URG4 monoclonal antibody
  • Hybridoma cell for resisting URG4 monoclonal antibody
  • Hybridoma cell for resisting URG4 monoclonal antibody

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Embodiment Construction

[0014] 1 Materials and Instruments

[0015] 1.1 Cell lines, tissue samples and animals

[0016] a. Myeloma cells (SP2 / 0) were obtained from the Department of Immunology, Fourth Military Medical University.

[0017] b. The cell lines HepG2-Pc, HepG2-HBx, 3T3, 3T3-URG4, MKN28 are preserved in our institute.

[0018] c. Tissue source: Cases operated in Xijing Hospital from 2002 to 2004, diagnosed by pathology.

[0019] d. BALB / c mice: Experimental Animal Center of Fourth Military Medical University.

[0020] 1.2 Main reagents

[0021] a. Freund's complete adjuvant, Freund's incomplete adjuvant, HAT, HT, ABTS, PEG (molecular weight 4000), anti-β-actin monoclonal antibody, diethyl pyrocarbonate (DEPC) and ethidium bromide (EB) : Sigma Corporation;

[0022] b. HRP-labeled rabbit anti-mouse IgG: Percin Company;

[0023] c. RPMI1640 medium: HyClone company;

[0024] d. Cellulose acetate membrane (NC): Hybond company;

[0025] e. Calf serum: Hangzhou Sijiqing Bioengineering Mat...

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Abstract

The invention mainly relates to the gene engineering, in particular to a hybridoma cell (1A8) which can stably secrete a URG4 monoclonal antibody and is a hybridoma cell strain with a preservation number of CGMCC NO.1908. The hybridoma cell strain with a preservation number of NO.1908 is used for secreting the hybridoma cell strain resisting the URG4 monoclonal antibody. The invention can stably secrete the hybridoma cell (1A8) for resisting the URG4 monoclonal antibody so as to further research the function of the URG4, and is used for the research of the cancer treatment.

Description

technical field [0001] The invention mainly relates to genetic engineering, especially a hybridoma cell (1A8) capable of stably secreting anti-URG4 monoclonal antibody. Background technique [0002] Current research shows that URG4 is a new gene that can be up-regulated by hepatitis B virus X protein (HBx) cloned by suppression subtractive hybridization and full-length polymerase chain reaction amplification technology (RACE-PCR). The full length of this gene is 3607 bases Base pairs, the coding region is 2769 base pairs, encoding a protein consisting of 922 amino acids and a molecular weight of 104KD. [0003] After comparison with the NIH Human Genome Database, it was located on human chromosome 7. According to the comprehensive protein information library analysis (SWISS PROT), this protein has a transmembrane helix (Transmembranehelices: 336-353); three nuclear localization signals (Nuclear localization signals: PYRGKRN 380, PPRRDKR 821, PRDKRQL 823); an ATP / GTP bindi...

Claims

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Application Information

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IPC IPC(8): C12N5/20C07K16/18
Inventor 刘杰
Owner NO 1 AUXILARY HOSPITAL NO 4 MILLITARY MEDICAL UNIV P L A
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