Method for exsomatizing screening grassiness salt resistsomatic mutation body

A technology of somatic mutation and screening method, which is applied in the field of in vitro screening of salt-tolerant somatic mutants of Elephant grass, can solve the problems of different seed maturation stages, easy shedding and germination rate, etc. Efficient effect

Active Publication Date: 2008-04-16
JIANGSU ACAD OF AGRI SCI
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Problems solved by technology

Although elephant grass is a perennial herb, it cannot bloom and bear fruit naturally in the middle and lower reaches of the Yangtze River in my country and the regions at the same latitude. asexual reproduction

Method used

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  • Method for exsomatizing screening grassiness salt resistsomatic mutation body

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Embodiment Construction

[0031] 1.1 Test materials

[0032] Elephant grass N51, publicly known and public, was introduced from the United States by the Jiangsu Academy of Agricultural Sciences in 1985, and the stems were asexually propagated and preserved. On April 20, 2005, the field was multiplied. With reference to the short-day treatment methods such as Chen Liwei, it began on June 20, 2005. After 3 to 4 weeks of short-day treatment of the target grass with black plastic film, the young panicle at the early booting stage was used as explants to inoculate on the callus induction medium, and white or light yellow, granular, embryogenic callus was induced. tissue as the test material.

[0033] 1.2 Medium

[0034] Minimal medium consisting of MS inorganic salts and B 5 Composed of organic ingredients, supplemented with 3% sucrose and 0.8% agar, pH 5.8. The callus induction medium is the basic medium supplemented with 4.0mg / L 2,4-D and 0.05mg / L kinetin (KT for short); the callus subculture medium is...

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Abstract

The invention relates to an screening method in vitro of elephant grass salt-tolerant somatic mutant belongs to a field of agricultural biotechnology. The process is: granular callus is transferred into proliferation medium with 16g / L NaCl, into differentiation medium with 16g / L NaCl after 45 days, and into rooting medium with no NaCl after 35 days to get regeneration plant; pot salt-tolerance identification of the regeneration plant is applied after tidal flat field comprehensive agronomic characters evaluation and grass-production measurement to get plants of which the fresh grass yield is higher than a control by 23.8% in tidal flat field with pH8.67-9.01; the fresh grass increment of the first time and the second tiome of cutting for the clonal progeny of the plants is higher than the control by 9.2% and 7.4% respectively in 1 / 2Hoagland nutrient solution with 8.25g / L sea-salt; therefore, the salt-tolerant somatic mutant of good comprehensive agronomic characters and significantly high salt-tolerance is selected. The invention has the advantages of simple-operation, short-cycle, and high selection efficiency.

Description

1. Technical field [0001] The invention relates to an in vitro screening method for salt-tolerant somatic cell mutants of Elephant grass, which belongs to the field of biotechnology and is specially used for engineering breeding of Elephant grass salt-tolerant cells. 2. Technical Background [0002] Elephant grass (Pennisetum purpureum Schumach), also known as purple pennisetum, is native to tropical Africa and was introduced into my country in the 1920s. A perennial grass especially suited to tropical and subtropical regions. Studies at home and abroad have shown that elephant grass is not only a high-quality green feed for grass-fed livestock and poultry, an ideal soil and water conservation crop for road slope protection, a high-quality raw material for medium and high-grade pulp and wood-based panels, but also an ideal biomass energy crop. Although elephant grass is a perennial herb, it cannot bloom and bear fruit naturally in the middle and lower reaches of the Yangtze ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01H4/00A01H1/06C12N5/04
Inventor 钟小仙佘建明顾洪如倪万潮张建丽丁成龙
Owner JIANGSU ACAD OF AGRI SCI
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