Reagent kit and method for judging normal person and liver cancer using magnetic bead supported matrix

A kit and matrix technology, applied in the field of protein detection, can solve the problem of no clinical liver cancer

Inactive Publication Date: 2008-04-30
许洋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, we found that there is no standardized kit for mass spectrometry di...

Method used

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  • Reagent kit and method for judging normal person and liver cancer using magnetic bead supported matrix
  • Reagent kit and method for judging normal person and liver cancer using magnetic bead supported matrix

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1 The distinction between normal and liver cancer and the preparation of mass spectrometry kits

[0073] (1) Experimental method

[0074] 1. Materials

[0075] Specimen source: Standardized quality control serum (plasma) preparation for mass spectrometry. The definitions meet the following criteria. The blood donors are half male and half male, and the blood type is O; the age is 18-30 years old; ethnicity, Han. The biochemical indicators were normal, including: total cholesterol, triglycerides, fasting blood sugar, hepatitis B surface antigen, liver function test, kidney function test; no family history of genetic diseases; no history of major infectious diseases. Women without pregnancy, men with no history of smoking. Fresh blood was drawn from 10 healthy subjects with blood type O (half male and half female), and centrifuged at 4°C immediately after the blood coagulated, at 10,000rpm, 4°C for 5min; sample processing and storage. Take 10 μl of mixed serum ...

Embodiment 2

[0100] The double-blind test of embodiment 2 kit

[0101] Several characteristic protein peaks 2043±15Da, 3936±15Da, 4469±15Da, 5080±15Da, 5318±15Da, 5932±15Da, 8687±15Da, 8938±15Da, 11490±15Da, 11700 were screened out from Example 1 ±15Da, 13130±15Da, 13756±15Da, double-blind test of 100 cases of normal and liver cancer ( figure 2 ).

[0102] double blind test

Liver cancer (100)

normal(100)

liver cancer

90

7

normal

10

93

[0103] Sensitivity 90% Specificity 93%

[0104] The experimental results using C8 and C18 hydrophobic matrix beads are consistent with the experimental results of the above-mentioned WCX anion matrix beads.

[0105] in conclusion

[0106] The kit of the method can distinguish between normal people and liver cancer, with a sensitivity of 90% and a specificity of 93%.

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Abstract

The invention relates to a proteome acquiring method which utilizes the method of the bead supporting stroma to capture the liver cancer protein group in the biology samples, and utilizes the magnetic separator to separate the bead and the sample without eccentric samples, and then utilizes the matter hidden method to analyze. The invention can be used in the protein fingerprint of the normal people and the liver cancer or the reagent box of the mass spectrum polypeptide atlas. The invention is accurate, convenient and quick.

Description

technical field [0001] The invention relates to a kit for a novel protein analysis method in liver cancer biological samples. One captures biomarkers via a protein-binding matrix and detects liver cancer biomarkers using quantitatively controlled mass spectrometry. The invention mentioned here relates to the field of protein detection and is a new non-invasive in vitro mass spectrometry detection method. The present invention can be applied to the detection method or kit of the liver cancer biomarker combination in the body fluid that has been separated from the human body. Background technique [0002] With the implementation and completion of the Human Genome Project, scientists proposed the concept of the post-genome (post-genome) project, and the focus of research shifted to functional genomics, and the main substance of biological function is protein. In 1994, Wilkins and Williams of Macquarie University in Australia first proposed the concept of proteome, which refer...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574G01N30/02G01N30/86G01N33/543G01N33/577
Inventor 许洋
Owner 许洋
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