DNA biosensor electrode manufacture method and uses thereof
A technology of biosensor and manufacturing method, which is applied in the manufacture of DNA biosensor electrodes and its application fields, can solve the problems of non-disposable use, difficult application and popularization, cumbersome processing, etc., and achieve simple manufacture, high sensitivity, and cumbersome solution The effect of the operation
Inactive Publication Date: 2012-01-04
EAST CHINA UNIV OF SCI & TECH
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Problems solved by technology
However, most of the current DNA biosensors are based on traditional gold electrodes, glassy carbon electrodes, etc., which are expensive, cumbersome to handle, and cannot be used at one time, so it is difficult to be applied and promoted.
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Embodiment 1
[0029] Utilize the DNA biosensor of the present invention to detect the characteristic DNA of pathogenic parahaemolyticus
[0030] 1: Fabrication of screen-printed electrodes
[0031] as attached figure 1 Shown includes: PVC bottom plate 1, conductive silver layer 2, carbon layer 3 or 3' (representing circle and square shape respectively), insulating layer 4, and disposable electrode 5 formed after printing. Prepare a PVC bottom plate, first clean the surface pollutants with ethanol solution, then rinse with double distilled water, and dry it for later use; use a screen printing machine to print a conductive silver layer on it, and dry it under an infrared lamp for 30~ 60min; then print a layer of carbon layer on it, mainly round and square, the size can be designed according to actual needs and then printed, and also dry under infrared light for 30-60min; then print a layer of insulating layer on it, dry After cutting into electrodes of the same size, they can be used as wo...
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The invention relates to a preparation method of DNA biosensor electrode and an application thereof, which can form a thin electrode by screen printing. The method comprises the following steps of: (a) selecting and processing a bottom plate; (b) printing a conductive Ag layer; (c) printing a carbon layer; and (d) printing an insulation layer. By adopting electrochemical deposition, the invention can achieve surface modification of screen-printed carbon electrode with zirconium oxide film in ZrOCl2 and KCl solution. SsDNA on the sensor can make intercross reaction with complementary-paired ssDNA in a hybridization solution to form dsDNA. Due to different actions between the single / double-chain DNA and methylene blue before and after hybridization, electrochemical signals emitted by the sensor in the methylene blue solution can be recorded by differential pulse voltammetry, and the DNA to be measured can be quantitatively and qualitatively measured by measuring the change of peak current. The method can be used for measuring DNA with a concentration lower than 10(-10) M, and is a technology with high sensitivity and specific selectivity. The preparation method has simple process, strong specificity and batch production, and has very important meanings in medical diagnosis, food industry, environment protection field, etc.
Description
technical field [0001] The invention relates to a preparation method and an application of a biosensor electrode, and relates to the preparation of a biosensor for detecting bacterial DNA by an electrochemical method. Background technique [0002] Food hygiene and quality issues have always attracted the attention of the general public, and the monitoring of harmful microorganisms in a large number of foods is an important part of food safety testing. At present, the general method for detecting bacteria is to identify the bacteria by measuring the physiological and morphological characteristics of the bacteria or by measuring the genetic composition of the bacteria. These related methods are complicated to operate and take a long time, and they are far from meeting the current requirements for microbial detection. In the past ten years, DNA biosensors have emerged, which have the advantages of rapidity, sensitivity, convenient operation, and no pollution. They have broad a...
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IPC IPC(8): G01N27/327
Inventor 蓝闽波张玲帆左少华滕渊洁赵艳会袁慧慧赵红莉孟宪江
Owner EAST CHINA UNIV OF SCI & TECH