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Drought resistance appraising method

An identification method and a technology of drought resistance, applied in the field of biology, can solve the problems of large measurement error, cumbersome measurement, and inability to measure, and achieve the effects of speeding up the breeding process, ensuring consistency, and high sensitivity

Inactive Publication Date: 2012-09-05
SHANGHAI AGROBIOLOGICAL GENE CENT
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Existing drought resistance measurement methods mainly use infrared detectors to measure canopy temperature. The measurement is relatively simple, but the canopy temperature is not stable enough, and it cannot be measured when there is wind in the field, and it is easily affected by microclimate conditions and population density.
[0007] At present, there are still drought resistance determination methods that use indicators such as nitrate reductase, plasma membrane permeability, and proline content to identify the drought resistance of materials. Although this physiological index can reflect the response of plants to drought stress, it is very cumbersome to measure. , and the measurement error is large, it is only suitable for the research of very few materials, and it is not suitable for group materials or more materials

Method used

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Experimental program
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Embodiment 1

[0031] A total of 17 recombinant inbred lines (RILs) were constructed with Zhenshan 97B (ZS97B) and IRAT109 as parents (numbered 3, 12, 37, 41, 49, 53, 74, 75, 89, 90, 108, 117, 187 and 19) and their parents as well as the identified variety Brazilian upland rice (IAPAR9) as the test objects, a total of 20 materials. Water treatment is carried out in the drought-resistance identification island established by the unit's Shanghai Chonggu Base. The identification island is surrounded by a 2m deep drainage ditch, and drainage holes of the same size are evenly distributed on the drainage ditch wall. Retractable plastic film is installed on the roof and surroundings, which can be opened on sunny days and put down on rainy days. Plant 4 rows of each material, 15 plants per row, carry out drought stress when 80% of the materials are in the early stage of panicle differentiation, cut off water for the materials near the ditch, keep the water in the ditch about 1.5m below the field, an...

Embodiment 2

[0036] The growth environment of the experimental material and the drought stress method are the same as in Example 1. Three typical paddy and upland rice varieties (ZS97B, IRAT109 and IAPAR9) have been tested for the water status of the plants throughout the day, and these three varieties have been measured every 1 to 1.5 hours. The ear water potential and leaf water potential values. The measurement results are shown in Table 2 and Figure 1-4 After the determination, rehydration was started, and then the seeds were harvested to investigate the yield and yield component factors. It can be seen from the measurement results that the order of drought resistance of the three varieties is: IRAT109>IAPAR9>ZS97B.

[0037] The instrument used in this embodiment to measure ear water potential and leaf water potential is a PMS Mode 11000 water potential meter produced in the United States.

[0038] From Table 2 and Figure 1-4 It can be seen that compared with leaf water potential, e...

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Abstract

The invention provides a draught-resistance performance identifying method which utilizes the detection of the water flow of spikelets for carrying out effective, simple and convenient draught-resistance performance identification and provides reliable identifying evidence and a method for plant, more particularly, for carrying out screening and identifying on the draught-resistance performance of rice. The draught-resistance performance identifying method can be used for the identification and breeding of rice draught-resistance materials and the research of draught-resistance mechanism, etc.

Description

technical field [0001] The invention belongs to the field of biology and relates to a method for identifying drought resistance, in particular to a method for identifying drought resistance of plants by measuring the water potential of ears. Background technique [0002] The shortage of water resources has become a global crisis. With population growth and urbanization, the water crisis will become even worse. Professor Brown pointed out that water resources are becoming an important factor restricting agricultural development in some countries, including China, and reducing agricultural water use, especially rice water use, has become the consensus of agricultural experts. [0003] Rice is one of the most important food crops in the world, the main source of food for 1 / 3 of the world's population, or 85% of the food mainly comes from rice. Rice production consumes a lot of water, accounting for 70% of agricultural water use. It takes about 5000L of water to produce 1kg of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/00
Inventor 罗利军刘国兰梅捍卫余新桥刘鸿艳
Owner SHANGHAI AGROBIOLOGICAL GENE CENT