Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

HIV-I resisting polypeptide, coded sequence and use thereof

A polypeptide and sequence technology, applied in the field of genetic engineering

Inactive Publication Date: 2012-05-23
TONGJI UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, the common problems of HIV inhibitor drugs are that the drugs are easy to be enzymatically hydrolyzed after entering the human body, HIV is easy to develop resistance to drugs, and immunogens are produced due to the large molecular weight of drugs. directly affect the efficacy of the drug

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] The gene synthesis of embodiment 1 combinatorial polypeptide

[0066] Referring to the preferred codons of Escherichia coli, chemically synthesized combined polypeptide C22 single-stranded nucleotide template (SEQ ID NO: 4) (5'GAA TTG GAT AAA TGG GCG TCG CTG TGG AAT TGG TTT AAT ATT ACC AAT TGG CTG TGG TATATT AAA3' ) and forward primer (SEQ ID NO:5) (5'GCG AAG CTT ATG GAA TTG GAT AAA TGG GCG3') and reverse primer (SEQ ID NO:6): 5'GCG GGA TCC TTA TTT AAT ATA CCA CAG CCA 3', the C22 gene was obtained by PCR reaction. C22-M3 (SEQ ID NO: 13), C22-CD4M9 (SEQ ID NO: 14), M3-CD4M9 (SEQ ID NO: 15), and C22-CD4M9-M3 (SEQ ID NO: 16) were all gene-generated according to this principle For synthesis, a restriction enzyme cutting site HindIII was introduced into the forward primer of each combination polypeptide, and a restriction enzyme cutting site Ecol I and a stop codon were designed in the reverse primer. M3 single-stranded nucleotide template (SEQ ID NO: 7) (CTT GGA GCA CTA C...

Embodiment 2

[0068] Example 2 Combination polypeptide plasmid construction

[0069] The PCR fragments obtained in Example 1 were double-digested with restriction endonucleases HindIII and BamHI respectively, and connected with the same double-digested carrier pTMHa30 (purchased from Amersham Company), the connected carrier (50 μl competent cells required 25ngDNA ) into competent Escherichia coli BL21(DE3), the volume should not exceed 5% of the competent cells, and the contents were mixed by gently swirling several times. Ice bath for 30 minutes; place the tube in a water bath at 42°C, and heat shock for 90 seconds; quickly transfer the tube to the ice bath for 90 seconds to cool the cells; add 800 μl LB medium to each tube, and shake slowly at 37°C for 45 minutes to revive the bacteria And express the antibiotic resistance marker gene encoded by the plasmid; centrifuge at low speed for 2 minutes, remove the supernatant, leave about 100 μl of medium in a microcentrifuge tube, and resuspend...

Embodiment 3

[0070] Embodiment 3: the expression of combination polypeptide of the present invention

[0071] Transform the recombinant plasmids obtained in Example 2 into E.coli BL21(DE3) respectively, pick single colonies and culture them overnight at 37°C, and transfer them to LB (containing kana 10 μg / ml) at 1% the next day, at 37°C Cultivate until the OD600 is about 0.6-0.8, add IPTG at a final concentration of 0.5mmol / L, continue to cultivate for 3-4 hours, induce the expression of the target protein, centrifuge, collect the bacteria, and after ultrasonic disruption, centrifuge again to collect the supernatant and precipitate. The expression sites of the target proteins were analyzed by SDS-PAGE (separating gel 15%, stacking gel 4.5%). The results indicated that the expression product was in the precipitate.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an anti-HIV-I polypeptide, a combination polypeptide, relative encoding sequences, expression carrier, a preparation method and application thereof, wherein the polypeptide and combination polypeptide are synthesized by gene recombination and expression or polypeptide synthesizer, with simple process, improved final yield, effect for inhibiting HIV-I intrusion, thus is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of genetic engineering, more specifically, the invention relates to a new anti-HIV-I polypeptide, its coding sequence and its application. Background technique [0002] Human immunodeficiency virus (human immunodeficiency virus, HIV) causes acquired immunodeficiency syndrome (acquired immunodeficiency syndrome, AIDS), namely AIDS. HIV belongs to RNA Retroviridae and can be divided into Type I (HIV-I) and Type II (HIV-II), among which HIV-I has a high infection rate and great harm. Infected people carry the virus all their lives, and after several years of incubation, they develop the disease, the immune system of the whole body is severely damaged, and eventually they die. Since the first AIDS case was discovered in New York, USA in 1981, AIDS has developed into a global epidemic because there is still no effective treatment for AIDS. It has seriously affected human health and social development, and has received ex...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435A61K38/17A61P31/18C12N15/12C12N15/63C07K16/18A61K39/00A61K39/12
Inventor 汪世龙史钧孙晓宇王玫何娇娟林楠王渊
Owner TONGJI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com