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Tissue culture method for fine stalk double butterflies

A technique of tissue culture and double butterfly, applied in the field of tissue culture of thin-stem double butterfly, can solve the problems of low seedling rate, difficult seed propagation, low germination rate of thin-stem double butterfly plants, etc., to increase antibacterial ability, overcome The effect of submultiplication is difficult

Inactive Publication Date: 2008-08-27
JISHOU UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved in the present invention is: aiming at the problems of low germination rate of the thin-stem double butterfly plant, low seedling rate after germination, and great difficulty in seed propagation, and establishing a high-efficiency tissue culture method of the thin-stem double butterfly

Method used

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Examples

Experimental program
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Embodiment Construction

[0020] Below in conjunction with example the present invention will be further described:

[0021] Step 1: Obtain sterile seedlings: take the rhizome of an annual plant, remove the leaves and petioles, cut into 6cm-long stems, wash with tap water and then rinse with saturated detergent solution for 5 minutes, then rinse with tap water for two hours , and then rinsed twice with distilled water, soaked in 75% alcohol on the ultra-clean workbench in the sterile room for 30s, and kept shaking to fully sterilize. Pour off the alcohol, add 0.1% mercuric chloride solution and a drop of Tween-80 to soak for 10 minutes for further disinfection. After pouring out the mercuric chloride solution, rinse it with sterile water for 6 times, place it on a dry sterile filter paper in a sterile petri dish, and absorb the surface moisture. Use a sharp knife to cut about 1.5cm in length, each section has a node with a budding stem segment, insert the lower end into the medium, the nodes and axill...

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Abstract

The invention provides a tissue culture method of the Tripterospermum filicaule, which belongs to the plant tissue culture method of the Tripterospermum and relates to the plant tissue culture method. The method includes four steps of obtaining and culturing aseptic seedling, propagation culturing, rooting culturing and transplanting. The culture mediums of the culture condition therein are all selected from L9 (3<4>) orthogonal tests and analysis of variance. (1) germinating the culture medium: MS+ZT 1.0mg.L-<1>; (2) proliferating the culture medium: MS+6BA 1.8mg.L-<1>; (3) rooting the culture medium: 1 / 2MS+IBa 0.8mg.L-<1>. The culture mediums are all added by 3% sucrose and 1.1% carrageenan with the PH value of 5.8 and the culture temperature at 25 plus or minus 1 DEG C. The automatic-controlled fluorescent lamp light source has a light intensity of 2000LX and a light duration of 14h.d<-1> and a replacement every 30d when proliferating. The method can increase the inductivity, the reproduction coefficient and the transplanting survival rate of the sprout, which solves the problems of the difficulty and the complication of the medical plant seed.

Description

technical field [0001] The invention relates to a method for tissue culture of the slender-stem double butterfly, which relates to the effective and rapid propagation, development and utilization of the medicinal plant. technical background [0002] There are very few literatures on the research on the genus Diptera. Domestic articles are mainly on classification and development. Foreign countries and Taiwan Province of my country have carried out research on the phytochemical components and pharmaceutical functional active components of the 5 species of the genus. Thin-stem double-butterfly plant, the seeds are small, the dormancy period is long, and the germination rate is extremely low. After three years of experiments, the applicant found that when the seeds of this plant were propagated, the seedlings actually had an ineffective juvenile stage, which could not be completed 6 months after germination. In the second leaf stage, not only the germination rate is extremely l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00C12N5/04
Inventor 田宏现李国民
Owner JISHOU UNIVERSITY
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