Method for improving hyaluronic acid volume of production of fermentation production by adding oxygen carrier dodecane
A technology of n-dodecane and hyaluronic acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., to achieve the effects of reducing production costs, increasing production, and reducing energy consumption
Inactive Publication Date: 2008-10-29
JIANGNAN UNIV
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Using the strategy of adding oxygen carriers to increase the prod
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A method for increasing the fermentation production of hyaluronic acid by adding an oxygen carrier of dodecane belongs to the technology field of applying the oxygen carrier to the optimization of the fermentation process. The invention adopts the method of adding the oxygen carrier of dedecane (1-5%, v/v) in a certain concentration to a fermentation broth after 8 hours' fermentation, so as to increase the dissolved oxygen concentration, thereby increasing the production of the hyaluronic acid. When the concentration of the dedecane is 5%, the production of the hyaluronic acid increases from 5.0g/L to 6.0g/L by contrast (no oxygen carrier is added). The method is simple, effective and applicable to the industrial production of the hyaluronic acid and has certain significance in guiding the optimization of other high-viscosity fermentation processes.
Description
A method for increasing the yield of fermented hyaluronic acid by adding oxygen carrier n-dodecane technical field A method for increasing the yield of hyaluronic acid produced by fermentation by adding an oxygen carrier n-dodecane relates to the technical field of application of an oxygen carrier addition strategy in fermentation process optimization. Background technique Hyaluronic acid is a high molecular weight mucopolysaccharide, and its two synthetic precursors are glucuronic acid and N-acetylglucosamine. Hyaluronic acid can be extracted from animal tissues such as rooster combs, or produced by microbial fermentation. Due to the low cost of the fermentation method and the high purity of the product, the microbial fermentation method is gradually replacing the tissue extraction method. At present, the research on the production of hyaluronic acid by microbial fermentation mainly focuses on the optimization of fermentation conditions, such as the optimization of the m...
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Inventor 陈坚堵国成王淼刘龙仇凯军
Owner JIANGNAN UNIV
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