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Hydroxy fatty acid oligomer and application in regulating calcium ion concentration in cell

A calcium ion and cell technology, applied in the field of hydroxy fatty acid oligomers, can solve the problems of high price

Inactive Publication Date: 2012-01-04
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But most of them are only used in the laboratory stage at present, and the price is expensive (Liu Daoming, 2000)

Method used

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  • Hydroxy fatty acid oligomer and application in regulating calcium ion concentration in cell
  • Hydroxy fatty acid oligomer and application in regulating calcium ion concentration in cell
  • Hydroxy fatty acid oligomer and application in regulating calcium ion concentration in cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 13

[0054] Example 13-Preparation of hydroxybutyric acid oligomer (OHB)

[0055] Accurately weigh 3g of poly-3-hydroxybutyric acid-4-hydroxybutyric acid (P3HB4HB), add 200ml of dichloromethane, and reflux at 90°C. After the PHB was completely dissolved, 0.3 g of p-toluenesulfonic acid and 0.5 g of water were added. After the reflux reaction was continued for 1 hour, 0.5 g of water was added. The reaction was terminated after 2 hours, filtered, precipitated with cold methanol, filtered, extracted with a Soxhlet extractor for 12 hours, and dried under vacuum at 50°C for 48 hours for later use. The number-average molecular weight (Mn) is 2000 as determined by gel permeation chromatography (GPC, Waters 1515), and nuclear magnetic resonance spectroscopy (NMR, Bruker 400) analysis product does not contain olefinic bonds and other cytotoxic substances.

Embodiment 2

[0056] Example 2 Preparation of oligomeric methyl 3-hydroxybutyrate (OHB-CH3)

[0057] Dissolve 1g PHB in 50mL chloroform, heat and reflux at 60°C for 2h to fully dissolve, add dropwise 50mL concentrated sulfuric acid methanol solution (10% v / v), and heat at 90°C to reflux for 2h. Add 50 mL of methanol pre-cooled at 4°C, let it stand for 1 hour, centrifuge the mixed solution at 8000 rpm and 4°C for 20 min, discard the supernatant, and add methanol to resuspend. Repeat the above steps twice and dry for 48 hours under vacuum at 50℃ for later use to obtain OHB-OCH 3 Solid white powder. The Mn was determined to be 2000 by gel permeation chromatography (GPC, Waters1515), and the nuclear magnetic resonance spectroscopy (NMR, Bruker400) analysis product did not contain olefinic bonds and other cytotoxic substances.

Embodiment 3

[0058] Example 3 Oligomeric 3-hydroxybutyrate-4-hydroxybutyrate methyl ester (O3HB4HB-CH 3 ) Preparation

[0059] Dissolve 1g P3HB4HB in 50mL chloroform, heat and reflux at 60°C for 2h to fully dissolve, add dropwise 50mL concentrated sulfuric acid methanol solution (10%v / v), and heat at 90°C to reflux for 2h. Add 50 mL of methanol pre-cooled at 4°C, let it stand for 1 hour, centrifuge the mixed solution at 8000 rpm and 4°C for 20 min, discard the supernatant, and add methanol to resuspend. Repeat the above steps twice and dry for 48 hours under vacuum at 50℃ for later use to obtain OHB-OCH 3 Solid white powder. Mn is 2100 detected by gel permeation chromatography (GPC, Waters 1515), and nuclear magnetic resonance spectroscopy (NMR, Bruker400) analysis product does not contain olefinic bonds and other cytotoxic substances.

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Abstract

The invention provides a hydroxy fatty acid oligomer and an application of the hydroxy fatty acid oligomer in adjusting the growth of cells and improving the concentration of calcium ions in the cells. As proved by experiments, the hydroxy fatty acid oligomer can inhibit the proliferation of cancer cells, induce the apoptosis and the death of the cells, prolong the G1 period of the cells and reduce the segmentation degree of the cancer cells. As shown by the further calcium imaging technical detection, the oligomer also can stimulate the increase of the concentration of dissociative calcium ions in the cells.

Description

Technical field [0001] The present invention relates to hydroxy fatty acid oligomers and their use in regulating the intracellular calcium ion concentration and regulating cell growth. Background technique [0002] Overview of the physiological functions of calcium [0003] In the human body, the calcium content exceeds 1000 grams. 99% of the calcium in the human body is deposited in the bones and teeth to promote their growth and development, maintain their shape and hardness; the remaining less than 10 grams are present in the blood and soft tissue cells It is precisely this small amount of calcium that plays a vital role in the process of life. At the end of the 19th century, the British cardiac physiologist Sydney Ringer (Sydney Ringer) realized calcium in an experiment studying the contractile function of the frog’s heart It is a special factor that can maintain heart function, and has since opened the prelude to the study of calcium ions (Sydney, 1884). By the 1960s, the me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61P3/14A61P9/04A61K31/25
Inventor 陈国强代忠伟孙杰
Owner SHANTOU UNIV
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