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Ferrous ion diagnosis/determination reagent kit and method for determining ferrous ion concentration

A technology of ferrous ion and kit, applied in ferrous ion diagnostic/determination kit, determination of ferrous ion concentration field

Inactive Publication Date: 2008-12-17
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there is still a reaction between the application of chromazurin S and high iron

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The ferrous ion diagnosis / determination reagent of the present embodiment is a single reagent, comprising:

[0040] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0041] Stabilizer 500mmol / L

[0042] Reduced coenzyme 0.25mmol / L

[0043] Cis-aconitic acid 10mmol / L

[0044] Coenzyme A 5mmol / L

[0045] Aconitase 6000U / L

[0046] Citric acid synthase 12000U / L

[0047] Malate dehydrogenase 18000U / L

[0048] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0049] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested ferrous ion sample to the reagent is 1 / 25. The reaction direction is negative reaction (falling reaction), the delay time is about 2 minutes, and th...

Embodiment 2

[0052] The ferrous ion diagnosis / determination reagent of the present embodiment is double reagent, comprises:

[0053] Reagent 1

[0054] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0055] Stabilizer 50mmol / L

[0056] Reduced coenzyme 0.25mmol / L

[0057] Cis-aconitic acid 10mmol / L

[0058] Coenzyme A 5mmol / L

[0059] Reagent 2

[0060] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0061] Stabilizer 500mmol / L

[0062]Aconitase 6000U / L

[0063] Citric acid synthase 12000U / L

[0064] Malate dehydrogenase 18000U / L

[0065] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0066] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume of the tested ferrous ion sample and reagent 1, reagent 2 The ratio i...

Embodiment 3

[0069] The ferrous ion diagnosis / determination reagent of the present embodiment is three reagents, comprises:

[0070] Reagent 1

[0071] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0072] Stabilizer 50mmol / L

[0073] Reduced coenzyme 0.25mmol / L

[0074] Cis-aconitic acid 10mmol / L

[0075] Coenzyme A 5mmol / L

[0076] Reagent 2

[0077] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0078] Stabilizer 500mmol / L

[0079] Citric acid synthase 12000U / L

[0080] Malate dehydrogenase 18000U / L

[0081] Reagent 3

[0082] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0083] Stabilizer 500mmol / L

[0084] Aconitase 6000U / L

[0085] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0086] When measuring the concentration of ferrous ions, set it on the automatic biochemical analyzer: temperature 37°C, reaction time 1...

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Abstract

The invention relates to a kit for diagnosing / mensurating ferrous ions by utilizing the technologies of the enzymic colorimetric method and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the ferrous ions, and belongs to the technology field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, cis-aconitic acid, coenzyme A, aconitase, citrate synthetase, malate dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, an enzymatic reactions occurs, then the reactant is placed under an ultraviolet / visible light analyzer, and the velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the ferrous ions.

Description

technical field [0001] The invention relates to a ferrous ion diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring the concentration of ferrous ions, which belongs to the technical field of medicine / food / environmental inspection and determination. Background technique [0002] Iron and total iron binding capacity are extremely important in human metabolism. Iron is an indispensable substance in the normal physiological process of the human body. The human body contains about 3-5g of iron, 70% of which is present in red blood cell hemoglobin; 25% is distributed in the mononuclear macrophage system (that is, the reticuloendothelial system, ferritin and hemosiderin in the liver, spleen, and bone marrow); 4.9% Distributed in myoglobin, cytochrome, iron-containing enzymes; 0.1% is iron in circulating plasma. [0003] Iron in the human body comes from food and is mainly absorbed in the duodenum and upper small intestine. Iron in ...

Claims

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Application Information

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IPC IPC(8): G01N33/84G01N21/31C12Q1/26C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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